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Sequence of CLR4_SCHPO

EC Number:2.1.1.366

EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
[histone H3]-N6,N6-dimethyl-lysine9 N-methyltransferase
O60016
Schizosaccharomyces pombe (strain 972 / ATCC 24843)
490
55918
Reaction
S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine9 = S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine9
Other sequences found for EC No. 2.1.1.366

General information:

Sequence
show sequence in fasta format
  0 MSPKQEEYEV ERIVDEKLDR NGAVKLYRIR WLNYSSRSDT WEPPENLSGC SAVLAEWKRR
 60 KRRLKGSNSD SDSPHHASNP HPNSRQKHQH QTSKSVPRSQ RFSRELNVKK ENKKVFSSQT
120 TKRQSRKQST ALTTNDTSII LDDSLHTNSK KLGKTRNEVK EESQKRELVS NSIKEATSPK
180 TSSILTKPRN PSKLDSYTHL SFYEKRELFR KKLREIEGPE VTLVNEVDDE PCPSLDFQFI
240 SQYRLTQGVI PPDPNFQSGC NCSSLGGCDL NNPSRCECLD DLDEPTHFAY DAQGRVRADT
300 GAVIYECNSF CSCSMECPNR VVQRGRTLPL EIFKTKEKGW GVRSLRFAPA GTFITCYLGE
360 VITSAEAAKR DKNYDDDGIT YLFDLDMFDD ASEYTVDAQN YGDVSRFFNH SCSPNIAIYS
420 AVRNHGFRTI YDLAFFAIKD IQPLEELTFD YAGAKDFSPV QSQKSQQNRI SKLRRQCKCG
480 SANCRGWLFG
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Sequence related references
Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
18378
Ivanova A.V.,Bonaduce M.J.,Ivanov S.V.,Klar A.J.S.
The chromo and SET domains of the Clr4 protein are essential for silencing in fission yeast.
Nat. Genet.
19
192-195
1998
18380
Wood V.,Gwilliam R.,Rajandream M.A.,Lyne M.H.,Lyne R.,Stewart A.,Sgouros J.G.,Peat N.,Hayles J.,Baker S.G.,Basham D.,Bowman S.,Brooks K.,Brown D.,Brown S.,Chillingworth T.,Churcher C.M.,Collins M.,Connor R.,Cronin A.,Davis P.,Feltwell T.,Fraser A.,Gentles S.,Goble A.,Hamlin N.,Harris D.E.,Hidalgo J.,Hodgson G.,Holroyd S.,Hornsby T.,Howarth S.,Huckle E.J.,Hunt S.,Jagels K.,James K.D.,Jones L.,Jones M.,Leather S.,McDonald S.,McLean J.,Mooney P.,Moule S.,Mungall K.L.,Murphy L.D.,Niblett D.,Odell C.,Oliver K.,O'Neil S.,Pearson D.,Quail M.A.,Rabbinowitsch E.,Rutherford K.M.,Rutter S.,Saunders D.,Seeger K.,Sharp S.,Skelton J.,Simmonds M.N.,Squares R.,Squares S.,Stevens K.,Taylor K.,Taylor R.G.,Tivey A.,Walsh S.V.,Warren T.,Whitehead S.,Woodward J.R.,Volckaert G.,Aert R.,Robben J.,Grymonprez B.,Weltjens I.,Vanstreels E.,Rieger M.,Schaefer M.,Mueller-Auer S.,Gabel C.,Fuchs M.,Duesterhoeft A.,Fritzc C.,Holzer E.,Moestl D.,Hilbert H.,Borzym K.,Langer I.,Beck A.,Lehrach H.,Reinhardt R.,Pohl T.M.,Eger P.,Zimmermann W.,Wedler H.,Wambutt R.,Purnelle B.,Goffeau A.,Cadieu E.,Dreano S.,Gloux S.,Lelaure V.,Mottier S.,Galibert F.,Aves S.J.,Xiang Z.,Hunt C.,Moore K.,Hurst S.M.,Lucas M.,Rochet M.,Gaillardin C.,Tallada V.A.,Garzon A.,Thode G.,Daga R.R.,Cruzado L.,Jimenez J.,Sanchez M.,del Rey F.,Benito J.,Dominguez A.,Revuelta J.L.,Moreno S.,Armstrong J.,Forsburg S.L.,Cerutti L.,Lowe T.,McCombie W.R.,Paulsen I.,Potashkin J.,Shpakovski G.V.,Ussery D.,Barrell B.G.,Nurse P.
The genome sequence of Schizosaccharomyces pombe.
Nature
415
871-880
2002
18381
Horn P.J.,Bastie J.-N.,Peterson C.L.
A Rik1-associated, cullin-dependent E3 ubiquitin ligase is essential for heterochromatin formation.
Genes Dev.
19
1705-1714
2005
18382
Jia S.,Kobayashi R.,Grewal S.I.S.
Ubiquitin ligase component Cul4 associates with Clr4 histone methyltransferase to assemble heterochromatin.
Nat. Cell Biol.
7
1007-1013
2005
18383
Ekwall K.,Ruusala T.
Mutations in rik1, clr2, clr3 and clr4 genes asymmetrically derepress the silent mating-type loci in fission yeast.
Genetics
136
53-64
1994
18384
Rea S.,Eisenhaber F.,O'Carroll D.,Strahl B.D.,Sun Z.-W.,Schmid M.,Opravil S.,Mechtler K.,Ponting C.P.,Allis C.D.,Jenuwein T.
Regulation of chromatin structure by site-specific histone H3 methyltransferases.
Nature
406
593-599
2000
18385
Bannister A.J.,Zegerman P.,Partridge J.F.,Miska E.A.,Thomas J.O.,Allshire R.C.,Kouzarides T.
Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain.
Nature
410
120-124
2001
18386
Nakayama J.,Rice J.C.,Strahl B.D.,Allis C.D.,Grewal S.I.S.
Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly.
Science
292
110-113
2001
18387
Hong E.J.,Villen J.,Gerace E.L.,Gygi S.P.,Moazed D.
A cullin E3 ubiquitin ligase complex associates with Rik1 and the Clr4 histone H3-K9 methyltransferase and is required for RNAi-mediated heterochromatin formation.
RNA Biol.
2
106-111
2005
18388
Matsuyama A.,Arai R.,Yashiroda Y.,Shirai A.,Kamata A.,Sekido S.,Kobayashi Y.,Hashimoto A.,Hamamoto M.,Hiraoka Y.,Horinouchi S.,Yoshida M.
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Nat. Biotechnol.
24
841-847
2006
18389
Zhang K.,Mosch K.,Fischle W.,Grewal S.I.
Roles of the Clr4 methyltransferase complex in nucleation, spreading and maintenance of heterochromatin.
Nat. Struct. Mol. Biol.
15
381-388
2008
18390
Gerace E.L.,Halic M.,Moazed D.
The methyltransferase activity of Clr4Suv39h triggers RNAi independently of histone H3K9 methylation.
Mol. Cell
39
360-372
2010
18391
Haldar S.,Saini A.,Nanda J.S.,Saini S.,Singh J.
Role of Swi6/HP1 self-association-mediated recruitment of Clr4/Suv39 in establishment and maintenance of heterochromatin in fission yeast.
J. Biol. Chem.
286
9308-9320
2011
18392
Zhang K.,Fischer T.,Porter R.L.,Dhakshnamoorthy J.,Zofall M.,Zhou M.,Veenstra T.,Grewal S.I.
Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA.
Science
331
1624-1627
2011
18393
Kusevic D.,Kudithipudi S.,Iglesias N.,Moazed D.,Jeltsch A.
Clr4 specificity and catalytic activity beyond H3K9 methylation.
Biochimie
135
83-88
2017
18394
Oya E.,Nakagawa R.,Yoshimura Y.,Tanaka M.,Nishibuchi G.,Machida S.,Shirai A.,Ekwall K.,Kurumizaka H.,Tagami H.,Nakayama J.I.
H3K14 ubiquitylation promotes H3K9 methylation for heterochromatin assembly.
EMBO Rep.
20
0-0
2019
18395
Horita D.A.,Ivanova A.V.,Altieri A.S.,Klar A.J.,Byrd R.A.
Solution structure, domain features, and structural implications of mutants of the chromo domain from the fission yeast histone methyltransferase Clr4.
J. Mol. Biol.
307
861-870
2001
18396
Min J.,Zhang X.,Cheng X.,Grewal S.I.S.,Xu R.M.
Structure of the SET domain histone lysine methyltransferase Clr4.
Nat. Struct. Biol.
9
828-832
2002
18397
Iglesias N.,Currie M.A.,Jih G.,Paulo J.A.,Siuti N.,Kalocsay M.,Gygi S.P.,Moazed D.
Automethylation-induced conformational switch in Clr4 (Suv39h) maintains epigenetic stability.
Nature
560
504-508
2018