Sequence of METF_ECOLI
EC Number:1.7.99.5
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
5,10-methylenetetrahydrofolate reductase (FADH2)
P0AEZ1
Escherichia coli (strain K12)
296
33103
Reaction
Other sequences found for EC No. 1.7.99.5
General information:
Sequence
0 MSFFHASQRD ALNQSLAEVQ GQINVSFEFF PPRTSEMEQT LWNSIDRLSS LKPKFVSVTY
60 GANSGERDRT HSIIKGIKDR TGLEAAPHLT CIDATPDELR TIARDYWNNG IRHIVALRGD
120 LPPGSGKPEM YASDLVTLLK EVADFDISVA AYPEVHPEAK SAQADLLNLK RKVDAGANRA
180 ITQFFFDVES YLRFRDRCVS AGIDVEIIPG ILPVSNFKQA KKFADMTNVR IPAWMAQMFD
240 GLDDDAETRK LVGANIAMDM VKILSREGVK DFHFYTLNRA EMSYAICHTL GVRPGL
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
1212873
Saint-Girons I.,Duchange N.,Zakin M.M.,Park I.,Margarita D.,Ferrara P.,Cohen G.N.
Nucleotide sequence of metF, the E. coli structural gene for 5-10 methylene tetrahydrofolate reductase and of its control region.
Nucleic Acids Res.
11
6723-6732
1983
1212874
Plunkett G. III,Burland V.,Daniels D.L.,Blattner F.R.
Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes.
Nucleic Acids Res.
21
3391-3398
1993
1212875
Blattner F.R.,Plunkett G. III,Bloch C.A.,Perna N.T.,Burland V.,Riley M.,Collado-Vides J.,Glasner J.D.,Rode C.K.,Mayhew G.F.,Gregor J.,Davis N.W.,Kirkpatrick H.A.,Goeden M.A.,Rose D.J.,Mau B.,Shao Y.
The complete genome sequence of Escherichia coli K-12.
Science
277
1453-1462
1997
1212876
Hayashi K.,Morooka N.,Yamamoto Y.,Fujita K.,Isono K.,Choi S.,Ohtsubo E.,Baba T.,Wanner B.L.,Mori H.,Horiuchi T.
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.
Mol. Syst. Biol.
2
0-0
2006
1212877
Katzen H.M.,Buchanan J.M.
Enzymatic synthesis of the methyl group of methionine. 8. Repression-derepression, purification, and properties of 5,10-methylenetetrahydrofolate reductase from Escherichia coli.
J. Biol. Chem.
240
825-835
1965
1212878
Zakin M.M.,Greene R.C.,Dautry-Varsat A.,Duchange N.,Ferrara P.,Py M.C.,Margarita D.,Cohen G.N.
Construction and physical mapping of plasmids containing the metJBLF gene cluster of E. coli K12.
Mol. Gen. Genet.
187
101-106
1982
1212879
Trimmer E.E.,Ballou D.P.,Ludwig M.L.,Matthews R.G.
Folate activation and catalysis in methylenetetrahydrofolate reductase from Escherichia coli: roles for aspartate 120 and glutamate 28.
Biochemistry
40
6216-6226
2001
1212880
Guenther B.D.,Sheppard C.A.,Tran P.,Rozen R.,Matthews R.G.,Ludwig M.L.
The structure and properties of methylenetetrahydrofolate reductase from Escherichia coli suggest how folate ameliorates human hyperhomocysteinemia.
Nat. Struct. Biol.
6
359-365
1999
1212881
Pejchal R.,Sargeant R.,Ludwig M.L.
Structures of NADH and CH3-H4folate complexes of Escherichia coli methylenetetrahydrofolate reductase reveal a spartan strategy for a ping-pong reaction.
Biochemistry
44
11447-11457
2005
1212882
Pejchal R.,Campbell E.,Guenther B.D.,Lennon B.W.,Matthews R.G.,Ludwig M.L.
Structural perturbations in the Ala --> Val polymorphism of methylenetetrahydrofolate reductase: how binding of folates may protect against inactivation.
Biochemistry
45
4808-4818
2006
1212883
Lee M.N.,Takawira D.,Nikolova A.P.,Ballou D.P.,Furtado V.C.,Phung N.L.,Still B.R.,Thorstad M.K.,Tanner J.J.,Trimmer E.E.
Functional role for the conformationally mobile phenylalanine 223 in the reaction of methylenetetrahydrofolate reductase from Escherichia coli.
Biochemistry
48
7673-7685
2009
