Sequence of O54666_AMYMD
EC Number:6.2.1.74
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
3-amino-5-hydroxybenzoate-[acyl-carrier protein] ligase
O54666
Amycolatopsis mediterranei
4735
495219
Reaction
ATP + 3-amino-5-hydroxybenzoate + a holo-[acyl-carrier protein] = 3-amino-5-hydroxybenzoyl-[acyl-carrier protein] + AMP + diphosphate
General information:
Sequence
show sequence in normal format
>O54666|3-amino-5-hydroxybenzoate-[acyl-carrier protein] ligase|EC 6.2.1.74|Amycolatopsis mediterranei|TrEMBL
MRTDLIKPLHVALLENATRFAGKPAFADDHRTVTYGDLEARTRRLAGHLAGLGVRHGDRV
AICLGNRVSTVESYFAILRAGAVGVPLNPGSATAELEHPLTDSGATVVVTDAAQAARLRL
APHVELLVTGDDVPEGAHSYDELALSEPAEPAADDLELDEPAWMFYTSGTTGRPKGVVST
QRNCLWSVASCYVPFPGLSDQDRVLWPLPLFHSLSHIACVLSATVVGASVRIADGSSADD
VMRLIEAESSTFLAGVPTTYHHLVRAARQRGFSAPSLRIGLAGGAVLGAGLRSEFEETFG
VPLIDAYGSTETCGAITMNPPDGARVEGSCGLAVPGVDVRVVDPDTGLDVPAGEEGEVWV
SGPNVMLGYHNSPEATAAAMRDGWFRTGDLARRDDAGYFTICGRIKELIIRGGANIHPGE
VEAVLRTVDGVADAAVGGVPHDTLGEVPVAYVIPGPTGFDPAALIEKCREQLSAYKVPDR
ILEVAHIPRTASGKIRRGLLTDEPAQLRYAATEHEEQSRHADESVAAALRARLSGLDERA
QCELLEDLVRTQAADVLGQPVPDGRAFRDLGFTSLAIVELRNRLTEHTGLWLPASAVFDH
PTPAALAARVRAELLGITQAVAEPVVAADPGEPIAIVGMACRLPGGVASPEDLWRLVAER
VDAVSEFPGDRGWDLDSLIDPDRERAGTSYVGQGGFLHDAGEFDAGFFGISPREAVAMDP
QQRLLLETSWEALENAGVDPIALKGTDTGVFSGLMGQGYGSGAVAPELEGFVTTGVASSV
ASGRVSYVLGLEGPAVTVDTACSSSLVAMHLAAQALRQGECSMALAGGVTVMATPGSFVE
FSRQRALAPDGRCKAFAAAADGTGWSEGVGVVVLERLSVARERGHRILAVLRGSAVNQDG
ASNGLTAPNGLSQQRVIRRALAAAGLAPSDVDVVEAHGTGTTLGDPIEAQALLATYGQER
KQPLWLGSLKSNIGHAQAAAGVAGVIKMVQALRHETLPPTLHVDKPTLEVDWSAGAIELL
TEARAWPRNGRPRRAGVSSFGVSGTNAHLILEEAPAEEPVAAPELPVVPLVVSARSTESL
SGQAERLASLLEGDVSLTEVAGALVSRRAVLDERAVVVAGSREEAVTGLRALNTAGSGTP
GKVVWVFPGQGTQWAGMGRELLAESPVFAERIAECAAALAPWIDWSLVDVLRGEGDLGRV
DVLQPACFAVMVGLAAVWESVGVRPDAVVGHSQGEIAAACVSGALSLEDAAKVVALRSQA
IAAELSGRGGMASVALGEDDVVSRLVDGVEVAAVNGPSSVVIAGDAHALDATLEILSGEG
IRVRRVAVDYASHTRHVEDIRDTLAETLAGISAQAPAVPFYSTVTSEWVRDAGVLDGGYW
YRNLRNQVRFGAAATALLEQGHTVFVEVSAHPVTVQPLSELTGDAIGTLRREDGGLRRLL
ASMGELFVRGIDVDWTAMVPAAGWVDLPTYAFEHRHYWLEPAEPASAGDPLLGTVVSTPG
SDRLTAVAQWSRRAQPWAVDGLVPNAALVEAAIRLGDLAGTPVVGELVVDAPVVLPRRGS
REVQLIVGEPGEQRRRPIEVFSREADEPWTRHAHGTLAPAAAAVPEPAAAGDATDVTVAG
LRDADRYGIHPALLDAAVRTVVGDDLLPSVWTGVSLLASGATAVTVTPTATGLRLTDPAG
QPVLTVESVRGTPFVAEQGTTDALFRVDWPEIPLPTAETADFLPYEATSAEATLSALQAW
LADPAETRLAVVTGDCTEPGAAAIWGLVRSAQSEHPGRIVLADLDDPAVLPAVVASGEPQ
VRVRNGVASVPRLTRVTPRQDARPLDPEGTVLITGGTGTLGALTARHLVTAHGVRHLVLV
SRRGEAPELQEELTALGASVAIAACDVADRAQLEAVLRAIPAEHPLTAVIHTAGVLDDGV
VTELTPDRLATVRRPKVDAARLLDELTREADLAAFVLFSSAAGVLGNPGQAGYAAANAEL
DALARQRNSLDLPAVSIAWGYWATVSGMTEHLGDADLRRNQRIGMSGLPADEGMALLDAA
IATGGTLVAAKFDVAALRATAKAGGPVPPLLRGLAPLPRRAAAKTASLTERLAGLAETEQ
AAALLDLVRRHAAEVLGHSGAESVHSGRTFKDAGFDSLTAVELRNRLAAATGLTLSPAMI
FDYPKPPALADHLRAKLFGSAANRPAEIGTAAAEEPIAIVAMACRFPGGVHSPEDLWRLV
ADGADAVTEFPADRGWDTDRLYHEDPDHEGTTYVRHGAFLDDAAGFDAAFFGISPNEALA
MDPQQRLLLETSWELFERAAIDPTTLAGQDIGVFAGVNSHDYSMRMHRAAGVEGFRLTGG
SASVLSGRVAYHFGVEGPAVTVDTACSSSLVALHMAVQALQRGECSMALAGGVMVMGTVE
TFVEFSRQRGLAPDGRCKAFADGADGTGWSEGVGLLLVERLSEAQRRGHQVLAVVRGSAV
NSDGASNGLTAPNGPSQQRVIRKALAAAGLSTSDVDAVEAHGTGTTLGDPIEAEALLATY
GQNRETPLWLGSVKSNLGHTQAAAGVAGVIKMVMAMRHGVLPRTLHVDRPSSYVDWSAGA
VELLTEARDWVSNGHPRRAGVSSFGIGGTNAHVVLEEVAAPITTPQPEPAEFLVPVLVSA
RTAAGLRGQAGRLAAFLGDRTDVRVPDAAYALATTRAQLDHRAVVLASDRAQLCADLAAF
GSGVVTGTPVDGKLAVLFTGQGSQWAGMGRELAETFPVFRDAFEAACEAVDTHLRERPLR
EVVFDDSALLDQTMYTQGALFAVETALFRLFESWGVRPGLLAGHSIGELAAAHVSGVLDL
ADAGELVAARGRLMQALPAGGAMVAVQATEDEVAPLLDGTVCVAAVNGPDSVVLSGTEAA
VLAVADELAGRGRKTRRLAVSHAFHSPLMEPMLDDFRAVAERLTYRAGSLPVVSTLTGEL
AALDSPDYWVGQVRNAVRFSDAVTALGAQGASTFLELGPGGALAAMALGTLGGPEQSCVA
TLRKNGAEVPDVLTALAELHVRGVGVDWTTVLDEPATAVGTVLPTYAFQHQRFWVDVDET
AAVSVTPPPAEPIVDRPVQDVLELVRESAAVVLGHRDAGSFDLDRSFKDHGFDSLSAVKL
RNRLRDFTGVELPSTLIFDYPNPAVLADHLRAELLGERPAAPAPVTRDVSDEPIAIVGMS
TRLPGGADSPEELWKLVAEGRDAVSGFPVDRGWDLDGLYHPDPAHAGTSYTRSGGFLHDA
AQFDAGLFGISPREALAMDPQQRLLLETSWEALERAGVDPLSARGSDVGVFTGIVHHDYV
TRLREVPEDVQGYTMTGTASSVASGRVAYVFGFEGPAVTVDTACSSSLVAMHLAAQALRQ
GECSMALAGGATVMASPDAFLEFSRQRGLSADGRCKAYAEGADGTGWAEGVGVVVLERLS
VARERGHRVLAVLRGSAVNQDGASNGLTAPNGPSQQRVIRGALASAGLAPSDVDVVEGHG
TGTALGDPIEVQALLATYGQEREQPLWLGSLKSNLGHTQAAAGVVGVIKMIMAMRHGVMP
ATLHVDERTSQVDWSAGAIEVLTEAREWPRTGRPRRAGVSSFGASGTNAHLIIEEGPAEE
AVDEEVASVVPLVVSARSAGSLAGQAGRLAAVLENESLAGVAGALVSGRATLNERAVVIA
GSRDEAQDGLQALARGENAPGVVTGTAGKPGKVVWVFPGQGSQWMGMGRDLLDSSPVFAA
RIKECAAALEQWTDWSLLDVLRGDADLLDRVDVVQPASFAMMVGLAAVWTSLGVTPDAVL
GHSQGEIAAACVSGALSLDDAAKVVALRSQAIAGELAGRGGMASVALSEEDAVARLTPWA
NRVEVAAVNSPSSVVIAGDAQALDEALEALAGDGVRVRRVAVDYASHTRHVEAIAETLAK
TLAGIDARVPAIPFYSTVLGTWIEQAVVDAGYWYRNLRQQVRFGPSVADLAGLGHTVFVE
ISAHPVLVQPLSEISDDAVVTGSLRRDDGGLRRLLASAAELYVRGVAVDWTAAVPAAGWV
DLPTYAFDRRHFWLHEAETAEAAEGMDGEFWTAIEQSDVDSLAELLELVPEQRGALSTVV
PVLAQWRDRRRERSTAEKLRYQVTWQPLEREAAGVPGGRWLAVVPAGTTDALLKELTGQG
LDIVRLEIEEASRAQLAEQLRNVLAEHDLTGVLSLLALDGGPADAAEITASTLALVQALG
DTTTSAPLWCLTSGAVNIGIQDAVTAPAQAAVWGLGRAVALERLDRWGGLVDLPAAIDAR
TAQALLGVLNGAAGEDQLAVRRSGVYRRRLVRKPVPESATSRWEPRGTVLVTGGAEGLGR
HASVWLAQSGAERLIVTGTDGVDELTAELAEFGTTVEFCADTDRDAIAQLVADSEVTAVV
HAADIAQTSSVDDTGVADLDEVFAAKVTTAVWLDQLFEDTPLDAFVVFSSIAGIWGGGGQ
GPAGAANAVLDALVEWRRARGLKATSIAWGALDQIGIGMDEAALAQLRRRGVIPMAPPLA
VTAMVQAVAGNEKAVAVADMDWAAFIPAFTSVRPSPLFADLPEAKAILRAAQDDGEDGDT
ASSLADSLRAVPDAEQNRILLKLVRGHASTVLGHSGAEGIGPRQAFQEVGFDSLAAVNLR
NSLHAATGLRLPATLIFDYPTPEALVGYLRVELLREADDGLDGREDDLRRVLAAVPFARF
KEAGVLDTLLGLADTGTEPGTDAETTEAAPAADDAELIDALDISGLVQRALGQTS
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
231093074
August P.R.,Tang L.,Yoon Y.J.,Ning S.,Mueller R.,Yu T.W.,Taylor M.,Hoffmann D.,Kim C.G.,Zhang X.,Hutchinson C.R.,Floss H.G.
Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699.
Chem. Biol.
5
69-79
1998
231093075
Kim C.G.,Yu T.W.,Fryhle C.B.,Handa S.,Floss H.G.
3-amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the formation of the precursor of mC7N units in rifamycin and related antibiotics.
J. Biol. Chem.
273
6030-6040
1998
231093077
Yu T.W.,Muller R.,Muller M.,Zhang X.,Draeger G.,Kim C.G.,Leistner E.,Floss H.G.
Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699.
J. Biol. Chem.
276
12546-12555
2001
