Sequence of THI4_ARATH
EC Number:2.4.2.60
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
cysteine-dependent adenosine diphosphate thiazole synthase
Q38814
Arabidopsis thaliana
349
36664
Reaction
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
Other sequences found for EC No. 2.4.2.60
General information:
Sequence
0 MAAIASTLSL SSTKPQRLFD SSFHGSAISA APISIGLKPR SFSVRATTAG YDLNAFTFDP
60 IKESIVSREM TRRYMTDMIT YAETDVVVVG AGSAGLSAAY EISKNPNVQV AIIEQSVSPG
120 GGAWLGGQLF SAMIVRKPAH LFLDEIGVAY DEQDTYVVVK HAALFTSTIM SKLLARPNVK
180 LFNAVAAEDL IVKGNRVGGV VTNWALVAQN HHTQSCMDPN VMEAKIVVSS CGHDGPFGAT
240 GVKRLKSIGM IDHVPGMKAL DMNTAEDAIV RLTREVVPGM IVTGMEVAEI DGAPRMGPTF
300 GAMMISGQKA GQLALKALGL PNAIDGTLVG NLSPELVLAA ADSAETVDA
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
1134064
Machado C.R.,de Oliveira R.L.,Boiteux S.,Praekelt U.M.,Meacock P.A.,Menck C.F.
Thi1, a thiamine biosynthetic gene in Arabidopsis thaliana, complements bacterial defects in DNA repair.
Plant Mol. Biol.
31
585-593
1996
1134065
Sato S.,Kotani H.,Nakamura Y.,Kaneko T.,Asamizu E.,Fukami M.,Miyajima N.,Tabata S.
Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones.
DNA Res.
4
215-230
1997
1134066
Cheng C.Y.,Krishnakumar V.,Chan A.P.,Thibaud-Nissen F.,Schobel S.,Town C.D.
Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.
Plant J.
89
789-804
2017
1134067
Yamada K.,Lim J.,Dale J.M.,Chen H.,Shinn P.,Palm C.J.,Southwick A.M.,Wu H.C.,Kim C.J.,Nguyen M.,Pham P.K.,Cheuk R.F.,Karlin-Newmann G.,Liu S.X.,Lam B.,Sakano H.,Wu T.,Yu G.,Miranda M.,Quach H.L.,Tripp M.,Chang C.H.,Lee J.M.,Toriumi M.J.,Chan M.M.,Tang C.C.,Onodera C.S.,Deng J.M.,Akiyama K.,Ansari Y.,Arakawa T.,Banh J.,Banno F.,Bowser L.,Brooks S.Y.,Carninci P.,Chao Q.,Choy N.,Enju A.,Goldsmith A.D.,Gurjal M.,Hansen N.F.,Hayashizaki Y.,Johnson-Hopson C.,Hsuan V.W.,Iida K.,Karnes M.,Khan S.,Koesema E.,Ishida J.,Jiang P.X.,Jones T.,Kawai J.,Kamiya A.,Meyers C.,Nakajima M.,Narusaka M.,Seki M.,Sakurai T.,Satou M.,Tamse R.,Vaysberg M.,Wallender E.K.,Wong C.,Yamamura Y.,Yuan S.,Shinozaki K.,Davis R.W.,Theologis A.,Ecker J.R.
Empirical analysis of transcriptional activity in the Arabidopsis genome.
Science
302
842-846
2003
1134068
Ribeiro A.,Praekelt U.,Akkermans A.D.L.,Meacock P.A.,van Kammen A.,Bisseling T.,Pawlowski K.
Identification of agthi1, whose product is involved in biosynthesis of the thiamine precursor thiazole, in actinorhizal nodules of Alnus glutinosa.
Plant J.
10
361-368
1996
1134069
Chabregas S.M.,Luche D.D.,Farias L.P.,Ribeiro A.F.,van Sluys M.A.,Menck C.F.,Silva-Filho M.C.
Dual targeting properties of the N-terminal signal sequence of Arabidopsis thaliana THI1 protein to mitochondria and chloroplasts.
Plant Mol. Biol.
46
639-650
2001
1134070
Chabregas S.M.,Luche D.D.,Van Sluys M.A.,Menck C.F.,Silva-Filho M.C.
Differential usage of two in-frame translational start codons regulates subcellular localization of Arabidopsis thaliana THI1.
J. Cell Sci.
116
285-291
2003
1134071
Papini-Terzi F.S.,Galhardo R.S.,Farias L.P.,Menck C.F.,Van Sluys M.A.
Point mutation is responsible for Arabidopsis tz-201 mutant phenotype affecting thiamin biosynthesis.
Plant Cell Physiol.
44
856-860
2003
1134072
Ribeiro D.T.,Farias L.P.,de Almeida J.D.,Kashiwabara P.M.,Ribeiro A.F.,Silva-Filho M.C.,Menck C.F.,Van Sluys M.A.
Functional characterization of the thi1 promoter region from Arabidopsis thaliana.
J. Exp. Bot.
56
1797-1804
2005
1134073
Zybailov B.,Rutschow H.,Friso G.,Rudella A.,Emanuelsson O.,Sun Q.,van Wijk K.J.
Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.
PLoS ONE
3
0-0
2008
1134074
Chatterjee A.,Abeydeera N.D.,Bale S.,Pai P.J.,Dorrestein P.C.,Russell D.H.,Ealick S.E.,Begley T.P.
Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase.
Nature
478
542-546
2011
1134075
Kolesinski P.,Piechota J.,Szczepaniak A.
Initial characteristics of RbcX proteins from Arabidopsis thaliana.
Plant Mol. Biol.
77
447-459
2011
1134076
Rapala-Kozik M.,Wolak N.,Kujda M.,Banas A.K.
The upregulation of thiamine (vitamin B1) biosynthesis in Arabidopsis thaliana seedlings under salt and osmotic stress conditions is mediated by abscisic acid at the early stages of this stress response.
BMC Plant Biol.
12
2-2
2012
1134077
Aryal U.K.,Krochko J.E.,Ross A.R.
Identification of phosphoproteins in Arabidopsis thaliana leaves using polyethylene glycol fractionation, immobilized metal-ion affinity chromatography, two-dimensional gel electrophoresis and mass spectrometry.
J. Proteome Res.
11
425-437
2012
1134078
Garcia A.F.,Dyszy F.,Munte C.E.,Demarco R.,Beltramini L.M.,Oliva G.,Costa-Filho A.J.,Araujo A.P.
THI1, a protein involved in the biosynthesis of thiamin in Arabidopsis thaliana: structural analysis of THI1(A140V) mutant.
Biochim. Biophys. Acta
1844
1094-1103
2014
1134079
Li C.L.,Wang M.,Wu X.M.,Chen D.H.,Lv H.J.,Shen J.L.,Qiao Z.,Zhang W.
THI1, a thiamine thiazole synthase, interacts with Ca2+-dependent protein kinase CPK33 and modulates the S-type anion channels and stomatal closure in Arabidopsis.
Plant Physiol.
170
1090-1104
2016
1134080
Godoi P.H.,Galhardo R.S.,Luche D.D.,Van Sluys M.A.,Menck C.F.,Oliva G.
Structure of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana.
J. Biol. Chem.
281
30957-30966
2006
