Sequence of CP27A_HUMAN

EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
cholestanetriol 26-monooxygenase
Q02318
Homo sapiens
531
60235
Reaction
(25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-al + 2 reduced adrenodoxin + 2 H+ + O2 = (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + 2 oxidized adrenodoxin + H2O
Sequences with same EC No.
Sequence
show sequence in fasta format
  0 MAALGCARLR WALRGAGRGL CPHGARAKAA IPAALPSDKA TGAPGAGPGV RRRQRSLEEI
 60 PRLGQLRFFF QLFVQGYALQ LHQLQVLYKA KYGPMWMSYL GPQMHVNLAS APLLEQVMRQ
120 EGKYPVRNDM ELWKEHRDQH DLTYGPFTTE GHHWYQLRQA LNQRLLKPAE AALYTDAFNE
180 VIDDFMTRLD QLRAESASGN QVSDMAQLFY YFALEAICYI LFEKRIGCLQ RSIPEDTVTF
240 VRSIGLMFQN SLYATFLPKW TRPVLPFWKR YLDGWNAIFS FGKKLIDEKL EDMEAQLQAA
300 GPDGIQVSGY LHFLLASGQL SPREAMGSLP ELLMAGVDTT SNTLTWALYH LSKDPEIQEA
360 LHEEVVGVVP AGQVPQHKDF AHMPLLKAVL KETLRLYPVV PTNSRIIEKE IEVDGFLFPK
420 NTQFVFCHYV VSRDPTAFSE PESFQPHRWL RNSQPATPRI QHPFGSVPFG YGVRACLGRR
480 IAELEMQLLL ARLIQKYKVV LAPETGELKS VARIVLVPNK KVGLQFLQRQ C
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Sequence related references
Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
1006060
Cali J.J.,Russell D.W.
Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis.
J. Biol. Chem.
266
7774-7778
1991
1006061
Guo Y.-D.,Strugnell S.,Back D.W.,Jones G.
Transfected human liver cytochrome P-450 hydroxylates vitamin D analogs at different side-chain positions.
Proc. Natl. Acad. Sci. U.S.A.
90
8668-8672
1993
1006063
Ota T.,Suzuki Y.,Nishikawa T.,Otsuki T.,Sugiyama T.,Irie R.,Wakamatsu A.,Hayashi K.,Sato H.,Nagai K.,Kimura K.,Makita H.,Sekine M.,Obayashi M.,Nishi T.,Shibahara T.,Tanaka T.,Ishii S.,Yamamoto J.,Saito K.,Kawai Y.,Isono Y.,Nakamura Y.,Nagahari K.,Murakami K.,Yasuda T.,Iwayanagi T.,Wagatsuma M.,Shiratori A.,Sudo H.,Hosoiri T.,Kaku Y.,Kodaira H.,Kondo H.,Sugawara M.,Takahashi M.,Kanda K.,Yokoi T.,Furuya T.,Kikkawa E.,Omura Y.,Abe K.,Kamihara K.,Katsuta N.,Sato K.,Tanikawa M.,Yamazaki M.,Ninomiya K.,Ishibashi T.,Yamashita H.,Murakawa K.,Fujimori K.,Tanai H.,Kimata M.,Watanabe M.,Hiraoka S.,Chiba Y.,Ishida S.,Ono Y.,Takiguchi S.,Watanabe S.,Yosida M.,Hotuta T.,Kusano J.,Kanehori K.,Takahashi-Fujii A.,Hara H.,Tanase T.-O.,Nomura Y.,Togiya S.,Komai F.,Hara R.,Takeuchi K.,Arita M.,Imose N.,Musashino K.,Yuuki H.,Oshima A.,Sasaki N.,Aotsuka S.,Yoshikawa Y.,Matsunawa H.,Ichihara T.,Shiohata N.,Sano S.,Moriya S.,Momiyama H.,Satoh N.,Takami S.,Terashima Y.,Suzuki O.,Nakagawa S.,Senoh A.,Mizoguchi H.,Goto Y.,Shimizu F.,Wakebe H.,Hishigaki H.,Watanabe T.,Sugiyama A.,Takemoto M.,Kawakami B.,Yamazaki M.,Watanabe K.,Kumagai A.,Itakura S.,Fukuzumi Y.,Fujimori Y.,Komiyama M.,Tashiro H.,Tanigami A.,Fujiwara T.,Ono T.,Yamada K.,Fujii Y.,Ozaki K.,Hirao M.,Ohmori Y.,Kawabata A.,Hikiji T.,Kobatake N.,Inagaki H.,Ikema Y.,Okamoto S.,Okitani R.,Kawakami T.,Noguchi S.,Itoh T.,Shigeta K.,Senba T.,Matsumura K.,Nakajima Y.,Mizuno T.,Morinaga M.,Sasaki M.,Togashi T.,Oyama M.,Hata H.,Watanabe M.,Komatsu T.,Mizushima-Sugano J.,Satoh T.,Shirai Y.,Takahashi Y.,Nakagawa K.,Okumura K.,Nagase T.,Nomura N.,Kikuchi H.,Masuho Y.,Yamashita R.,Nakai K.,Yada T.,Nakamura Y.,Ohara O.,Isogai T.,Sugano S.
Complete sequencing and characterization of 21,243 full-length human cDNAs.
Nat. Genet.
36
40-45
2004
1006065
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
Genome Res.
14
2121-2127
2004
1006066
Leitersdorf E.,Reshef A.,Meiner V.,Levitzki R.,Schwartz S.P.,Dann E.J.,Berkman N.,Cali J.J.,Klapholz L.,Berginer V.M.
Frameshift and splice-junction mutations in the sterol 27-hydroxylase gene cause cerebrotendinous xanthomatosis in Jews or Moroccan origin.
J. Clin. Invest.
91
2488-2496
1993
1006067
Pikuleva I.A.,Babiker A.,Waterman M.R.,Bjoerkhem I.
Activities of recombinant human cytochrome P450c27 (CYP27) which produce intermediates of alternative bile acid biosynthetic pathways.
J. Biol. Chem.
273
18153-18160
1998
1006068
Pikuleva I.A.,Puchkaev A.,Bjoerkhem I.
Putative helix F contributes to regioselectivity of hydroxylation in mitochondrial cytochrome P450 27A1.
Biochemistry
40
7621-7629
2001
1006069
Bodin K.,Andersson U.,Rystedt E.,Ellis E.,Norlin M.,Pikuleva I.,Eggertsen G.,Bjoerkhem I.,Diczfalusy U.
Metabolism of 4 beta -hydroxycholesterol in humans.
J. Biol. Chem.
277
31534-31540
2002
1006070
Shinkyo R.,Sakaki T.,Kamakura M.,Ohta M.,Inouye K.
Metabolism of vitamin D by human microsomal CYP2R1.
Biochem. Biophys. Res. Commun.
324
451-457
2004
1006071
Heo G.Y.,Bederman I.,Mast N.,Liao W.L.,Turko I.V.,Pikuleva I.A.
Conversion of 7-ketocholesterol to oxysterol metabolites by recombinant CYP27A1 and retinal pigment epithelial cells.
J. Lipid Res.
52
1117-1127
2011
1006072
Bian Y.,Song C.,Cheng K.,Dong M.,Wang F.,Huang J.,Sun D.,Wang L.,Ye M.,Zou H.
An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
J. Proteomics
96
253-262
2014
1006073
Mast N.,Anderson K.W.,Lin J.B.,Li Y.,Turko I.V.,Tatsuoka C.,Bjorkhem I.,Pikuleva I.A.
Cytochrome P450 27A1 Deficiency and Regional Differences in Brain Sterol Metabolism Cause Preferential Cholestanol Accumulation in the Cerebellum.
J. Biol. Chem.
292
4913-4924
2017
1006074
Cali J.J.,Hsieh C.-L.,Francke U.,Russell D.W.
Mutations in the bile acid biosynthetic enzyme sterol 27-hydroxylase underlie cerebrotendinous xanthomatosis.
J. Biol. Chem.
266
7779-7783
1991
1006075
Kim K.-K.,Kubota S.,Kuriyama M.,Fujiyama J.,Bjorkhem I.,Eggertsen G.,Seyama Y.
Identification of new mutations in sterol 27-hydroxylase gene in Japanese patients with cerebrotendinous xanthomatosis (CTX).
J. Lipid Res.
35
1031-1039
1994
1006076
Chen W.,Kubota S.,Kim K.-S.,Cheng J.,Kuriyama M.,Eggertsen G.,Bjorkhem I.,Seyama Y.
Novel homozygous and compound heterozygous mutations of sterol 27-hydroxylase gene (CYP27) cause cerebrotendinous xanthomatosis in three Japanese patients from two unrelated families.
J. Lipid Res.
38
870-879
1997
1006077
Chen W.,Kubota S.,Ujike H.,Ishihara T.,Seyama Y.
A novel arg362ser mutation in the sterol 27-hydroxylase gene (CYP27): its effects on pre-mRNA splicing and enzyme activity.
Biochemistry
37
15050-15056
1998
1006078
Lamon-Fava S.,Schaefer E.J.,Garuti R.,Salen G.,Calandra S.
Two novel mutations in the sterol 27-hydroxylase gene causing cerebrotendinous xanthomatosis.
Clin. Genet.
61
185-191
2002