Sequence of LIPA_ECOLI
EC Number:2.8.1.8
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
Reaction
[protein]-N6-(octanoyl)-L-lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = [protein]-N6-[(R)-dihydrolipoyl]-L-lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin
Other sequences found for EC No. 2.8.1.8
General information:
Sequence
0 MSKPIVMERG VKYRDADKMA LIPVKNVATE REALLRKPEW MKIKLPADST RIQGIKAAMR
60 KNGLHSVCEE ASCPNLAECF NHGTATFMIL GAICTRRCPF CDVAHGRPVA PDANEPVKLA
120 QTIADMALRY VVITSVDRDD LRDGGAQHFA DCITAIREKS PQIKIETLVP DFRGRMDRAL
180 DILTATPPDV FNHNLENVPR IYRQVRPGAD YNWSLKLLER FKEAHPEIPT KSGLMVGLGE
240 TNEEIIEVMR DLRRHGVTML TLGQYLQPSR HHLPVQRYVS PDEFDEMKAE ALAMGFTHAA
300 CGPFVRSSYH ADLQAKGMEV K
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
54113
Hayden M.A.,Huang I.,Bussiere D.E.,Ashley G.W.
The biosynthesis of lipoic acid. Cloning of lip, a lipoate biosynthetic locus of Escherichia coli.
J. Biol. Chem.
267
9512-9515
1992
54114
Reed K.E.,Cronan J.E. Jr.
Lipoic acid metabolism in Escherichia coli: sequencing and functional characterization of the lipA and lipB genes.
J. Bacteriol.
175
1325-1336
1993
54115
Oshima T.,Aiba H.,Baba T.,Fujita K.,Hayashi K.,Honjo A.,Ikemoto K.,Inada T.,Itoh T.,Kajihara M.,Kanai K.,Kashimoto K.,Kimura S.,Kitagawa M.,Makino K.,Masuda S.,Miki T.,Mizobuchi K.,Mori H.,Motomura K.,Nakamura Y.,Nashimoto H.,Nishio Y.,Saito N.,Sampei G.,Seki Y.,Tagami H.,Takemoto K.,Wada C.,Yamamoto Y.,Yano M.,Horiuchi T.
A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.
DNA Res.
3
137-155
1996
54117
Blattner F.R.,Plunkett G. III,Bloch C.A.,Perna N.T.,Burland V.,Riley M.,Collado-Vides J.,Glasner J.D.,Rode C.K.,Mayhew G.F.,Gregor J.,Davis N.W.,Kirkpatrick H.A.,Goeden M.A.,Rose D.J.,Mau B.,Shao Y.
The complete genome sequence of Escherichia coli K-12.
Science
277
1453-1462
1997
54118
Hayashi K.,Morooka N.,Yamamoto Y.,Fujita K.,Isono K.,Choi S.,Ohtsubo E.,Baba T.,Wanner B.L.,Mori H.,Horiuchi T.
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.
Mol. Syst. Biol.
2
0-0
2006
54119
Miller J.R.,Busby R.W.,Jordan S.W.,Cheek J.,Henshaw T.F.,Ashley G.W.,Broderick J.B.,Cronan J.E. Jr.,Marletta M.A.
Escherichia coli LipA is a lipoyl synthase: in vitro biosynthesis of lipoylated pyruvate dehydrogenase complex from octanoyl-acyl carrier protein.
Biochemistry
39
15166-15178
2000
54120
Zhao X.,Miller J.R.,Jiang Y.,Marletta M.A.,Cronan J.E. Jr.
Assembly of the covalent linkage between lipoic acid and its cognate enzymes.
Chem. Biol.
10
1293-1302
2003
54121
Cicchillo R.M.,Iwig D.F.,Jones A.D.,Nesbitt N.M.,Baleanu-Gogonea C.,Souder M.G.,Tu L.,Booker S.J.
Lipoyl synthase requires two equivalents of S-adenosyl-L-methionine to synthesize one equivalent of lipoic acid.
Biochemistry
43
6378-6386
2004
54122
Cicchillo R.M.,Lee K.-H.,Baleanu-Gogonea C.,Nesbitt N.M.,Krebs C.,Booker S.J.
Escherichia coli lipoyl synthase binds two distinct [4Fe-4S] clusters per polypeptide.
Biochemistry
43
11770-11781
2004
54124
Choi-Rhee E.,Cronan J.E.
A nucleosidase required for in vivo function of the S-adenosyl-L-methionine radical enzyme, biotin synthase.
Chem. Biol.
12
589-593
2005
54125
Cicchillo R.M.,Booker S.J.
Mechanistic investigations of lipoic acid biosynthesis in Escherichia coli: both sulfur atoms in lipoic acid are contributed by the same lipoyl synthase polypeptide.
J. Am. Chem. Soc.
127
2860-2861
2005
