EC Number   |
Protein Variants |
Reference |
|---|
   7.3.2.1 | D188K |
no ATP hydrolyzing ability |
-, 659162 |
| 7.3.2.1 | D228E |
drastic increase in expression compared to wild-type, mutant shows no transport activity |
711256 |
| 7.3.2.1 | D382A |
Km: (phosphate) 0.057 mM (in the presence of 25 mM NaCl), 0.49 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 156 nmol/mg of protein/h, results indicate that Arg-228 may participate in interactions associated with protein conformational changes required for full transport activity |
711256 |
| 7.3.2.1 | D382E |
Km: (phosphate) 0.046 mM (in the presence of 25 mM NaCl), 0.73 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 96 nmol/mg of protein/h, drastic increase in expression compared to wild-type, results indicate that Arg-228 may participate in interactions associated with protein conformational changes required for full transport activity |
711256 |
| 7.3.2.1 | D382N |
mutant shows no transport activity |
711256 |
| 7.3.2.1 | DELTA1-375 |
mutant lacking the entire N-terminal SPX domain shows no differences in protein level or plasma membrane localisation compared to wild-type, phosphate uptake-rate is higher compared to wild-type, Km remains in the same range as wild-type, truncated mutant has increased catalytic activity |
711852 |
| 7.3.2.1 | E240Q |
mutation in pstC, loss of phosphate transport through the pst system, alkaline phosphatase activity remains repressed |
289157 |
| 7.3.2.1 | G48I |
mutation in pstB, loss of phosphate transport through the Pst system and derepression of alkaline phosphatase activity |
289157 |
| 7.3.2.1 | K49Q |
mutation in pstB, loss of phosphate transport through the Pst system and derepression of alkaline phosphatase activity |
289157 |
| 7.3.2.1 | more |
construction of deletion mutants, mutation of either pstS and phnD leads to impaired cell growth in minimal medium, the cells are only viable in high phosphate concentrated medium |
-, 670171 |
