EC Number |
Protein Variants |
Reference |
---|
6.3.5.9 | D45N |
less than 0.2% of amidation activity with glutamine and with NH4+, compared to wild-type values |
661083 |
6.3.5.9 | D48N |
less than 0.3% of amidation activity with glutamine and with NH4+, compared to wild-type values |
661083 |
6.3.5.9 | D97N |
32.4% of wild-type amidation activity with glutamine, 16.5% of wild-type amidation activity with NH4+ |
661083 |
6.3.5.9 | E90Q |
less than 0.02% of amidation activity with glutamine and with NH4+, compared to wild-type values |
661083 |
6.3.5.9 | L47A |
10.6% of wild-type amidation activity with glutamine, 44% of wild-type amidation activity with NH4+, mutation specifically decreases the affinity of the enzyme to the cobyrinic acid c-monoamide |
661083 |
6.3.5.9 | Y46A |
3.8% of wild-type amidation activity with glutamine, 2% of wild-type amidation activity with NH4+, mutation specifically decreases the affinity of the enzyme to the cobyrinic acid c-monoamide |
661083 |