EC Number   |
Protein Variants |
Reference |
|---|
  6.3.2.29 | C595S |
enhanced catalytic activity |
-, 713897 |
| 6.3.2.29 | E856A |
site-directed mutagenesis, inactive mutant |
691115 |
| 6.3.2.29 | E856V |
site-directed mutagenesis, inactive mutant |
-, 691115 |
| 6.3.2.29 | more |
construction of truncation mutant CphANE1?C45, which lacks up to 45 amino acids at its C-terminus, the mutant retains full enzymatic activity and produced polymers, however, the removal of one additional amino acid, Glu856, results in complete inactivation of CphANE1?C46, phenotype, overview. Removal of the sequence Leu867-Leu870 results in dramatically decreased thermostability |
-, 691115 |
| 6.3.2.29 | more |
two truncated CphAs, lacking 31 (CphANE1del96) or 59 (CphANE1del180) amino acids of the C-terminal region, are derived from cphANE1 by deleting 96 or 180 bp from its 3' region through the introduction of stop codons. In comparison to the wild-type gene, cphANE1del96 conferrs about 2.1-2.2fold higher enzyme activity on Escherichia coli. These cells accumulate about twofold more cyanophycin than cells expressing the wild-type gene |
671478 |
