EC Number   |
Protein Variants |
Reference |
|---|
    6.2.1.25 | A227G |
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme, overview |
744341 |
| 6.2.1.25 | H334A |
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme, overview |
744341 |
| 6.2.1.25 | I335A |
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme, overview |
744341 |
| 6.2.1.25 | K427A |
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme, overview |
744341 |
| 6.2.1.25 | K427A |
site-directed mutagenesis, the mutant shows substrate specificity compared to the wild-type enzyme, overview |
744341 |
| 6.2.1.25 | L333A |
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme, overview |
744341 |
| 6.2.1.25 | more |
an enzyme cascade reaction using a benzoate CoA ligase (BadA), a modified nonribosomal peptide synthase (PheAT), a phenylpropanoyltransferase (BAPT), and a benzoyltransferase (NDTNBT) to produce an anticancer paclitaxel analogue and its precursor from the commercially available biosynthetic intermediate baccatin III is established. This reaction network has great potential for optimization to scale-up production and is attractive in its regioselective O- and N-acylation steps that remove protecting group manipulations used in paclitaxel analogue synthesis |
744372 |
| 6.2.1.25 | more |
insertional mutation in gene xabE, using suicide-vector pJP-XabE, leads to complete loss of albicidin production ability. The mutant fails to induce symptoms of leaf scald in maize plants, in contrast to wild-type Xanthomonas albilineans, which elicits characteristic white pencil line symptoms in inoculated maize leaves |
-, 713869 |
| 6.2.1.25 | more |
mutational analysis and modification to expand the substrate specificity substantially compared to that of wild-type BadA, overview. Activities are achieved for substrates with larger substituents, including phenyl acetate. The Lys427 nonconserved residue is essential for the thiolation step of BadA, but not adenylation. Variously acylated CoAs can serve as substrates of acyl CoA-dependent acyltransferases in coupled enzyme assays to produce analogues of bioactive natural products |
744341 |
