EC Number |
Protein Variants |
Reference |
---|
6.1.1.23 | G83K |
aspartylation reaction of the mutant enzyme is 55% as fast as the wild-type enzyme |
659070 |
6.1.1.23 | G83K |
site-directed mutagenesis, the mutation increases the specificity of tRNAAsp charging over that of tRNAAsn by 4.2fold |
659070 |
6.1.1.23 | H26A |
site-directed mutagenesis, mutation the amino acid located in the AspRS anticodon binding domain limits the specificity of this nondiscriminating enzyme towards tRNAAsn, altered tRNA substrate specificity compared to the wild-type enzyme, overview |
-, 677106 |
6.1.1.23 | H26A/P84A |
site-directed mutagenesis, mutation the amino acid located in the AspRS anticodon binding domain limits the specificity of this nondiscriminating enzyme towards tRNAAsn, altered tRNA substrate specificity compared to the wild-type enzyme, overview |
677106 |
6.1.1.23 | H26A/P84K |
site-directed mutagenesis, mutation the amino acid located in the AspRS anticodon binding domain limits the specificity of this nondiscriminating enzyme towards tRNAAsn, altered tRNA substrate specificity compared to the wild-type enzyme, overview |
677106 |
6.1.1.23 | H26Q |
site-directed mutagenesis, mutation the amino acid located in the AspRS anticodon binding domain limits the specificity of this nondiscriminating enzyme towards tRNAAsn, altered tRNA substrate specificity compared to the wild-type enzyme, overview |
-, 677106 |
6.1.1.23 | H26Q/P84A |
site-directed mutagenesis, mutation the amino acid located in the AspRS anticodon binding domain limits the specificity of this nondiscriminating enzyme towards tRNAAsn, altered tRNA substrate specificity compared to the wild-type enzyme, overview |
-, 677106 |
6.1.1.23 | H26Q/P84K |
site-directed mutagenesis, mutation the amino acid located in the AspRS anticodon binding domain limits the specificity of this nondiscriminating enzyme towards tRNAAsn, altered tRNA substrate specificity compared to the wild-type enzyme, overview |
677106 |
6.1.1.23 | H28Q |
wild-type enzyme shows a slight preference to tRNAAsn over tRNAAsp. Mutation H28Q leads to a reverse tRNA preference |
659475 |
6.1.1.23 | H31L |
aspartylation reaction of the mutant enzyme is 84% as fast as the wild-type enzyme |
659070 |