EC Number |
Protein Variants |
Reference |
---|
5.5.1.9 | E29V |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 0%, wild-type: 100%. Relative activity: below 0.5% (wild-type: 100%) |
729167 |
5.5.1.9 | G108E |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 0%, wild-type: 100%. Relative activity: below 0.5% (wild-type: 100%). Km (cycloeucalenol) increased compared to wild-type |
729167 |
5.5.1.9 | D260V |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 0%, wild-type: 100%. Relative activity: below 1.5% (wild-type: 100%) |
729167 |
5.5.1.9 | E29K |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 1.7%, wild-type: 100%. Relative activity: below 0.5% (wild-type: 100%) |
729167 |
5.5.1.9 | G28E |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 15%, wild-type: 100%. Relative activity: 1% (wild-type: 100%) |
729167 |
5.5.1.9 | D260Y |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 22%, wild-type: 100%. Relative activity: 0.7% (wild-type: 100%) |
729167 |
5.5.1.9 | E29H |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 33%, wild-type: 100%. Relative activity: 3.5% (wild-type: 100%). Km (cycloeucalenol) decreased compared to wild-type |
729167 |
5.5.1.9 | F116L |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 37%, wild-type: 100%. Relative activity: 4.7% (wild-type: 100%) |
729167 |
5.5.1.9 | G28V |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 46%, wild-type: 100%. Relative activity: 1.6% (wild-type: 100%) |
729167 |
5.5.1.9 | W112K |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 47%, wild-type: 100%. Relative activity: 1.8% (wild-type: 100%) |
729167 |