EC Number   |
Protein Variants |
Reference |
|---|
  5.4.99.20 | D79N |
inactive mutant enzyme |
706734 |
| 5.4.99.20 | D79T |
inactive mutant enzyme |
706734 |
| 5.4.99.20 | more |
generation of the deletion mutants RluE DELTAN(1-35) lacking the N-terminal region deleted at residue 35 and RluE DELTAR158-K161 lacking loop L7-8. Mutant RluE DELTAN(1-35) shows pseudouridylation activity similar to wild-type, while the activity of mutant RluE DELTAR158-K161 is significantly reduced |
-, 748400 |
| 5.4.99.20 | R153A/P155G/R158A/R160A |
site-directed mutagenesis, the mutant shows reduced activity compared to wild-type |
-, 748400 |
| 5.4.99.20 | R196A |
site-directed mutagenesis, the mutant shows reduced activity compared to wild-type |
-, 748400 |
| 5.4.99.20 | R31A/R32A/K33A |
site-directed mutagenesis, the mutant shows pseudouridylation activity similar to wild-type |
-, 748400 |
| 5.4.99.20 | R60A/K61A |
site-directed mutagenesis, the mutant shows reduced activity compared to wild-type |
-, 748400 |
