EC Number   |
Protein Variants |
Reference |
|---|
   5.4.99.17 | A306F |
the mutant produces apolypodatetraene, gamma-polypodatetraene and neopolypoda-5(6),13,17,21-teraene from squalene and polypoda-8(9),13,17,21-tetraen-3a-ol, polypoda-7(8),13,17,21-tetraen-3alpha-ol and polypoda-7(8),13,17,21-tetraen-3beta-ol from a racemic mixture of (3S)-2,3-oxidosqualene and (3R)-2,3-oxidosqualene |
747552 |
| 5.4.99.17 | A306T |
the mutant produces (20R)-dammara-13(17),24-dien-3a-ol, (20R)-dammara-13(17),24-dien-3b-ol, (20R)-dammara-12(13),24-dien-3a-ol, (20R)-dammara-12(13),24-dien-3b-ol and (20R,24R)-17-epi-epoxydammarane from a racemic mixture of (3S)-2,3-oxidosqualene and (3R)-2,3-oxidosqualene |
747552 |
| 5.4.99.17 | A306V |
the mutant produces apolypodatetraene, dammara-13(17)-24-diene, gamma-polypodatetraene, (17E)(13aH)-malabarica-14(27),17,21-triene, (20R)-dammara-12(13),24-diene, (20R)-eupha-7(8),24-diene, (20S)-tirucalla-7(8),24-diene, and 17-epi-dammara-20(21),24-diene from squalene |
747552 |
| 5.4.99.17 | A419F |
inactive |
748730 |
| 5.4.99.17 | C25S/C50S/C435S/C455S/C537S |
quintuple mutant |
662757 |
| 5.4.99.17 | C25S/C50S/D376C/C435S/C455S/C537S |
sextuple mutant |
662757 |
| 5.4.99.17 | C435S/D374I/D374V/H451F |
inactive mutant |
726715 |
| 5.4.99.17 | C435S/D374I/D374V/H451F |
site-directed mutagenesis, inactive mutant |
726715 |
| 5.4.99.17 | D376C |
inactive |
747564, 747626, 748730 |
| 5.4.99.17 | D376E |
10% enzyme activity |
440045 |
