EC Number   |
Protein Variants |
Reference |
|---|
    5.4.99.16 | A255P |
2.0% activity compared to the wild type enzyme |
715072 |
| 5.4.99.16 | D112A |
site-directed mutagenesis, inactive mutant |
-, 727233 |
| 5.4.99.16 | D294A |
site-directed mutagenesis, inactive mutant |
727233 |
| 5.4.99.16 | D403A |
site-directed mutagenesis, inactive mutant |
-, 727233 |
| 5.4.99.16 | D411P |
3.2% activity compared to the wild type enzyme |
715072 |
| 5.4.99.16 | D41P |
99.6% activity compared to the wild type enzyme |
715072 |
| 5.4.99.16 | E330A |
site-directed mutagenesis, residue E330 is vital for product formation, the mutant shows only hydrolase activity but no transglucosidic activity |
-, 746863 |
| 5.4.99.16 | E338A |
site-directed mutagenesis, inactive mutant |
-, 727233 |
| 5.4.99.16 | E469P |
50% activity compared to the wild type enzyme |
715072 |
| 5.4.99.16 | F115A |
site-directed mutagenesis, inactive mutant |
-, 727233 |
