EC Number   |
Protein Variants |
Reference |
|---|
    5.4.2.11 | A60S |
69% of wild-type activity |
652790 |
| 5.4.2.11 | E86Q |
1% of wild-type activity |
652790 |
| 5.4.2.11 | E93D |
3D structure in solution obtained with NMR |
652846 |
| 5.4.2.11 | E93D |
less than 1% of wild-type activity |
651088 |
| 5.4.2.11 | H181A |
His181 is substituted by Ala in site-directed mutagenesis. The resulting enzyme has a reduced activity and still requires 2,3-diphosphoglycerate. His181 seems to be important for cofactor-binding |
3230 |
| 5.4.2.11 | K100A |
no covalent modification by inhibitor 1-benzyl 4-[(3R,4R,6R)-6-[1-(hex-5-ynoyloxy)-1-methylethyl]-1-oxaspiro[2.5]oct-4-yl] (2R)-2-(2,3-dihydro-1H-indol-3-ylmethyl)butanedioate |
681851 |
| 5.4.2.11 | K246G |
mutant enzymes in which Ser11 is replaced by Gly, i.e. S11G and Lys246 by Gly, i.e. K246G do not have significantly altered kinetic values |
3231 |
| 5.4.2.11 | L245G |
39% of wild-type mutase activity, 111% of wild-type phosphatase activity |
649656 |
| 5.4.2.11 | L245G/L246G |
105% of wild-type mutase activity, 111% of wild-type phosphatase activity |
649656 |
| 5.4.2.11 | L246G |
98% of wild-type mutase activity, 122% of wild-type phosphatase activity |
649656 |
