EC Number   |
Protein Variants |
Reference |
|---|
    5.3.3.8 | D149E |
decrease in catalytic activity |
678385 |
| 5.3.3.8 | D149E |
turnover number for trans-3-hexenoyl-CoA is 2.3fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme |
678385 |
| 5.3.3.8 | DELTAH10 |
inactive |
746625 |
| 5.3.3.8 | E134Q |
catalytic properties of the five different component enzyme activities of the alpha/Arg134Gln mutant complex show no significant changes as compared with those of the wild-type complex. In the alpha/Glu139Gln mutant complex DELTA3-cis-DELTA2-trans-enoyl-CoA isomerase shows 60% decreased turnover number and a significant increase in Km for 3-cis-tetradecenoyl-CoA |
3012 |
| 5.3.3.8 | E136A |
variant to study the importance of Glu136 for catalysis |
662630 |
| 5.3.3.8 | E139Q |
The replacement of Glu165 by Gln leads to a strongly reduced enzymatic activity. Tyr150 is not involved in isomerase catalysis |
3012 |
| 5.3.3.8 | E151X |
site-directe mutagenesis of putative active-site amino acid residues. Exchange of Arg151 and Asp211 leads to a reduced expression of the recombinant enzyme accompanied by a reduced activity. The replacement of Glu165 by Gln leads to a strongly reduced enzymatic activity. Tyr150 is not involved in isomerase catalysis |
3026 |
| 5.3.3.8 | E165Q |
site-directe mutagenesis of putative active-site amino acid residues. Exchange of Arg151 and Asp211 leads to a reduced expression of the recombinant enzyme accompanied by a reduced activity. The replacement of Glu165 by Gln leads to a strongly reduced enzymatic activity. Tyr150 is not involved in isomerase catalysis |
3026 |
| 5.3.3.8 | F268A |
the mutant shows reduced activity compared to the wild type enzyme |
746625 |
| 5.3.3.8 | K242C |
decrease in catalytic activity, mutants shows additional hydratase activity on 2-hexenoyl-CoA with Vmax of 1.9 micromol per min and mg |
678385 |
