EC Number   |
Protein Variants |
Reference |
|---|
    5.2.1.1 | C194A |
inactive |
-, 715258 |
| 5.2.1.1 | C194S |
the mutant displays more than 1000fold reduced turnover compared to the wild type enzyme |
-, 715258 |
| 5.2.1.1 | C200A |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme, substrate-binding structure: maleate can be found to exist inside a cavity of the enzyme, totally surrounded by the protein |
-, 728334 |
| 5.2.1.1 | C76S |
the mutant displays more than 1000fold reduced turnover compared to the wild type enzyme |
-, 715258 |
| 5.2.1.1 | C84A |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme |
-, 728334 |
| 5.2.1.1 | M138C |
mutant enzyme shows 90% of the activity of the wild-type enzyme |
-, 650608 |
| 5.2.1.1 | M18C |
mutant enzyme shows 30% of the activity of the wild-type enzyme |
-, 650608 |
| 5.2.1.1 | M201C |
mutant enzyme shows 90% of the activity of the wild-type enzyme |
650608 |
| 5.2.1.1 | M46C |
mutant enzyme shows 30% of the activity of the wild-type enzyme |
-, 650608 |
| 5.2.1.1 | M57C |
mutant enzyme shows 40% of the activity of the wild-type enzyme |
-, 650608 |
