EC Number   |
Protein Variants |
Reference |
|---|
   5.1.3.30 | more |
enzyme overexpression for heterologous production of D-psicose from D-fructose, under the optimal conditions, the equilibrium ratio of D-fructose to D-psicose is 69:31. For high production of D-psicose, 216 g/L D-psicose are produced with 28.8% turnover yield at pH 6.5 and 55°C. The recombinant DPEase exhibits weak acid stability and thermostability and has a high affinity and turnover for the substrate D-fructose |
-, 746879 |
| 5.1.3.30 | more |
high-level intra- and extra-cellular production of D-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis, fed-batch fermentation in 7.5 l fermentor, overview |
748350 |
| 5.1.3.30 | more |
immobilization of recombinant extracellular enzyme DPE onto anion exchange resin D301, DPE immobilization yield is 90%. D-Psicose is produced using the immobilized enzyme, separated by simulated moving bed chromatography (SMB), and purified by crystallization. The purity of the D-psicose crystals reaches 99.1%, method optimization, overview |
748289 |
| 5.1.3.30 | more |
on the basis of the Izumoring strategy, D-allulose can be obtained from D-glucose by coupling D-glucose isomerase (GIase) and DPEase, with D-fructose as the intermediate. In this reaction system, D-fructose is firstly converted from D-glucose by GIase, and immediately isomerised to D-allulose by DPEase. Recombinant co-expression with GIase from Acidothermus cellulolyticus from plasmid pETDuet-Dosp-DPE/Acce-GI, optimization of the biotransformation consitions, method, overview. When the reactions reaches equilibrium under optimal conditions,the equilibrium ratio of D-glucose, D-fructose and D-allulose is approximately 6.5:7:3, respectively. The transformation rate is about 18%. The optimum pH of the Acce-GI/Dosp-DPE co-expression system is lower than that of the BGI/RDPE co-expression system, while the optimum temperature is higher than that of the BGI/RDPE co-expression system |
748501 |
| 5.1.3.30 | more |
to improve the expression level of D-psicose 3-epimerase, the DPEase gene is fused with the promoter P43 to generate an expression cassette that introduced an identical cohesive end, and the resultant P43-DPE expression cassette is used as a monomeric fragment. The tandem repeats of the expression cassette are constructed by the isocaudamer method and integrated into the amyE gene locus of Bacillus subtilis by double-crossover. After DPEase is expressed in Bacillus subtilis, the antibiotic resistance gene is deleted via the Cre/lox system, thus generating food-grade strains. D-Psicose (D-allulose) production by freeze-dried enzyme powder, the optimal substrate concentration is 300 g/L of D-fructose, generating 26.67 g/l/h of D-allulose yield with an 8.89% conversion rate |
-, 748007 |
