EC Number |
Protein Variants |
Reference |
---|
5.1.3.20 | D210N |
mutant display activity similar to that of the wild type |
672239 |
5.1.3.20 | E210G |
site-directed mutagenesis, structure compared to the wild-type enzyme |
-, 726590 |
5.1.3.20 | K178M |
mutant has severely compromised epimerase activities that are more than 3 orders of magnitude lower than that of the wild type |
672239 |
5.1.3.20 | K178M |
site-directed mutagenesis, structure compared to the wild-type enzyme |
-, 726590 |
5.1.3.20 | K208M |
mutant display activity similar to that of the wild type |
672239 |
5.1.3.20 | more |
construction of the mutant Escherichia coli strains WBB06 and WJW00, that can synthesize Kdo2-lipid A, by deleting the rfaD gene from the genome of Escherichia coli wild-type strain W3110. 3-Deoxy-D-manno-oct-2-ulosonic acid (Kdo)2-lipid A is the conserved structure domain of lipopolysaccharide found in most Gram-negative bacteria, and is believed to stimulate the human innate immune system through the TLR4/MD2 complex. Kdo2-lipid A is an important stimulator for studying the mechanism of the innate immune system and for developing bacterial vaccine adjuvants. Compared with the wild-type strain W3110, WJW00 shows increased hydrophobicity, higher cell permeability, greater autoaggregation and decreased biofilm-forming ability |
-, 748527 |
5.1.3.20 | Y140F |
mutant has severely compromised epimerase activities that are more than 3 orders of magnitude lower than that of the wild type |
672239 |
5.1.3.20 | Y140F |
the mutant shows 0.08% epimerase activity compared to the wild type enzyme |
716848 |