  4.4.1.2 | up |
induction of the enzyme is mainly sulfur amino-acid-dependent, especially methionine and homocysteine. The induction of the enzyme in response to various amino acids is evaluated, and the results reveal that sulfur-containing amino acids strongly induce Hcy gamma-lyase production by the isolate when compared with other amino acids. The maximum enzyme productivity is detected in the presence of methionine (1.8 U/mg), homocysteine (1.2 U/mg), cysteine (1.23 U/mg), and asparagine (1.2 U/mg). Some enzyme activity is also detected when using various non-sulfur amino acids as substrates, such as glutamine, alanine, and phenylalanine, indicating the multifunctional deaminating catalytic activity of the crude enzyme. The lowest enzyme activity is detected in the presence of tyrosine and lysine |
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