EC Number   |
Protein Variants |
Reference |
|---|
    4.3.1.17 | A65G |
site-directed mutagenesis of the human liver isozyme cSDH, large structural alterations, serine binding is not affected, overview |
691069 |
| 4.3.1.17 | A65S |
structure of A65S hSDH mutant is determined at 1.3 A resolution. Mutant shows decreased activity (50%) with substrate L-serine compared to wild-type. Mutant shows measurable activity with substrate D-serine |
728723 |
| 4.3.1.17 | A65S/V225S |
double mutant shows only 10% of activity with substrate L-serine compared to wild-type (100%). Double mutant shows very little but measurable activity with substrate D-serine |
728723 |
| 4.3.1.17 | C183A |
135% of wild-type activity. Enzyme retains the positive cooperativity seen in the native enzyme |
713970 |
| 4.3.1.17 | C221A |
70% of wild-type activity. Enzyme retains the positive cooperativity seen in the native enzyme |
713970 |
| 4.3.1.17 | C226A |
100% of wild-type activity. Enzyme retains the positive cooperativity seen in the native enzyme |
713970 |
| 4.3.1.17 | C303A |
site-directed mutagenesis of the human liver isozyme cSDH, structural alterations, overview |
691069 |
| 4.3.1.17 | C304A |
10% of wild-type activity. Mutant displays altered kinetics with a higher Km and a Hill coefficient indicating negative homotropic cooperativity |
713970 |
| 4.3.1.17 | C309A |
site-directed mutagenesis of the cancer cell isozyme cSDH, structural alterations, overview |
691069 |
| 4.3.1.17 | C343A |
inactive, mutants lacks the charge transfer absorbance at 400 nm that is indicative of the 4Fe-4S center |
713970 |
