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EC Number
Protein Variants
Commentary
Reference
4.2.99.20
D128A
conserved among MenH proteins
702326
4.2.99.20
D210A
decreased activity
685225
4.2.99.20
D210A
kcat/Km is 9091fold lower than wild-type value
685225
4.2.99.20
D210A
part of the catalytically essential triad
702326
4.2.99.20
F153A
residue involved in open-closed transition, mutation leads to large decrease in enzymatic activity
730042
4.2.99.20
H232A
decreased activity
685225
4.2.99.20
H232A
kcat/Km is 208333fold lower than wild-type value
685225
4.2.99.20
H232A
part of the catalytically essential triad
702326
4.2.99.20
H232K
part of the catalytically essential triad
702326
4.2.99.20
K212A
conserved among MenH proteins
702326
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