EC Number   |
Protein Variants |
Reference |
|---|
   4.2.2.2 | C132I/C156N/C194L |
mutations increase expression level |
649194 |
| 4.2.2.2 | D106N |
mutant enzyme without pectate lyase activity |
651067 |
| 4.2.2.2 | D107N |
complete loss of activity, crystallization data |
-, 746624 |
| 4.2.2.2 | D125 |
loss of catalytic activity |
-, 747884 |
| 4.2.2.2 | D126N |
the ratio of turnover number to Km-value is 57.5% of the wild-type value |
651067 |
| 4.2.2.2 | D147 |
loss of catalytic activity |
-, 747884 |
| 4.2.2.2 | D154E |
mutant enzyme with 44% of the activity of the wild-type enzyme, the Km-value for the substrate lime pectin (with 75% methyl esterification) is 1.2fold higher than the Km-value of the wild-type enzyme |
653654 |
| 4.2.2.2 | D154N |
mutant enzyme with 44% of the activity of the wild-type enzyme, the Km-value for the substrate lime pectin (with 75% methyl esterification) is 2.3fold higher than the Km-value of the wild-type enzyme. The pH-optimum is higher than that of the wild-type enzyme |
653654 |
| 4.2.2.2 | D173A |
the mutation results in approximately 40% of wild type activity |
702380 |
| 4.2.2.2 | D173A/N180A/K247A |
the mutation results in approximately 0.2% of wild type activity |
702380 |
