EC Number |
Protein Variants |
Reference |
---|
4.2.1.90 | H281N |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme |
696299 |
4.2.1.90 | H282N |
site-directed mutagenesis |
696299 |
4.2.1.90 | H329N |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme |
696299 |
4.2.1.90 | H33N |
site-directed mutagenesis, inactive mutant |
696299 |
4.2.1.90 | more |
construction of a DELTAlraC knockout mutant from strain CBS 141257, gene deletion and transcriptomic analysis is performed to elucidate the function of LraC, protoplast-mediated transformations of Aspergillus niger. Deletion of genes lraA, lraB or lraC causes a reduction in expression level of the other genes of the L-rhamnose pathway. Strongly reduced expression levels of lraA, lraB, rhaR, rglB, rgxA and rgaeA are observed in the DELTAlraC mutant upon the 2 h transfer to L-rhamnonate compared to the reference strain. But none of the deletions of genes lraA, lraB or lraC results in accumulation of the inducer of the pathway regulator (RhaR) |
-, 747418 |
4.2.1.90 | more |
introduction of genes LRA1-4 or LRA1-3, LRA5 and LAR6 compensats for the L-rhamnose-defective phenotype of an Escherichia coli mutant |
697897 |