EC Number   |
Protein Variants |
Reference |
|---|
    4.2.1.83 | C124S |
kcat/Km for 4-oxalomesaconate is 1.9fold higher than wild-type value, mutant enzyme is less stable than wild-type enzyme at 4°C in 20 mM potassium phosphate buffer containing 1 mM DTT, mutant enzyme is less stable than wild-type enzyme at 4°C in 20 mM potassium phosphate buffer containing 1 mM DTT |
678816 |
| 4.2.1.83 | C131S |
kcat/Km for 4-oxalomesaconate is 26% higher than wild-type value, mutant enzyme is less stable than wild-type enzyme at 4°C in 20 mM potassium phosphate buffer containing 1 mM DTT |
-, 678816 |
| 4.2.1.83 | C183S |
kcat/Km for 4-oxalomesaconate is 1.8fold higher than wild-type value, mutant enzyme is less stable than wild-type enzyme at 4°C in 20 mM potassium phosphate buffer containing 1 mM DTT |
-, 678816 |
| 4.2.1.83 | C186S |
kcat/Km for 4-oxalomesaconate is 16% higher than wild-type value, mutant enzyme is more stable than wild-type enzyme at 4°C in 20 mM potassium phosphate buffer containing 1 mM DTT |
678816 |
| 4.2.1.83 | C205S |
kcat/Km for 4-oxalomesaconate is 29% higher than wild-type value, mutant enzyme is less stable than wild-type enzyme at 4°C in 20 mM potassium phosphate buffer containing 1 mM DTT |
678816 |
| 4.2.1.83 | C260S |
kcat/Km for 4-oxalomesaconate is 18% higher than wild-type value, mutant enzyme is less stable than wild-type enzyme at 4°C in 20 mM potassium phosphate buffer containing 1 mM DTT |
-, 678816 |
| 4.2.1.83 | C92S |
kcat/Km for 4-oxalomesaconate is 18% higher than wild-type value, mutant enzyme is less stable than wild-type enzyme at 4°C in 20 mM potassium phosphate buffer containing 1 mM DTT |
-, 678816 |
| 4.2.1.83 | C96S |
kcat/Km for 4-oxalomesaconate is 3.9fold lower than wild-type value, mutant enzyme is less stable than wild-type enzyme at 4°C in 20 mM potassium phosphate buffer containing 1 mM DTT |
-, 678816 |
| 4.2.1.83 | more |
generation of several pmdE gene truncated mutants, lacking residues 6 to 226, 32 to 296, or 105 to 215, overview. A pmdE knock out mutant loses the ability to grow on vanillate, 3-hydroxybenzoate, and 4-hydroxybenzoate as carbon sources. This ability to grow on vanillate, 3-hydroxybenzoate, and 4-hydroxybenzoate is restored by genetic complementation |
-, 726779 |
