EC Number   |
Protein Variants |
Reference |
|---|
   4.2.1.66 | D275E |
site-directed mutagenesis, 0-29% reduced activity compared to the wild-type enzyme |
651378, 651610 |
| 4.2.1.66 | F170L |
no activity |
746898 |
| 4.2.1.66 | F170L |
site-directed mutagenesis, inactive mutant compared to the wild-type enzyme |
651378, 651610 |
| 4.2.1.66 | K136R |
site-directed mutagenesis, 52% reduced activity compared to the wild-type enzyme |
651378, 651610 |
| 4.2.1.66 | K136R/D275E/V281A/M302S |
mutant enzyme shows lower activity |
746898 |
| 4.2.1.66 | M302S |
site-directed mutagenesis, 22% reduced activity compared to the wild-type enzyme |
651378, 651610 |
| 4.2.1.66 | more |
REMI technique is used to construct mutants with improved cyanide-degradation ability from biocontrol fungus Trichoderma koningii strain T30. Transformants TkA9 (0.00553 mM formamide formed per h and mg protein) from T30 and Th64 (5.35 mM formamide formed per h and mg protein) from T21 had higher cyanide hydratase activity than other transformants and their wild strains |
-, 678755 |
| 4.2.1.66 | S13A |
site-directed mutagenesis, 19-47% reduced activity compared to the wild-type enzyme |
651378, 651610 |
| 4.2.1.66 | T12P |
site-directed mutagenesis, inactive mutant |
651378, 651610 |
| 4.2.1.66 | T12Q |
site-directed mutagenesis, 10-48% reduced activity compared to the wild-type enzyme |
651378, 651610 |
