EC Number |
Protein Variants |
Reference |
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4.2.1.165 | more |
bchF deletion mutant, mutant phenotype with altered pigment biosynthesis, detailed overview |
-, 748684 |
4.2.1.165 | more |
deletion of gene bchF in a BChl b-producing strain of Rhodobacter sphaeroides leads to the production of an analogue of bacteriochlorophyllide g, BChl gP, where P is phytyl, rather than the native BChl aP. In the bchF-deletion mutant, hydration of the C3-vinyl group in 3-vinyl bacteriochlorophyllide a is blocked, but this precursor can be esterified with phytol. Enzyme BchF deletion- in the DELTAbciA/bchXYZBv background results in formation of BChl g esterified with phytol (BChl gP) |
747177 |
4.2.1.165 | more |
reconstitution of a bacteriochlorophyll formation pathway consisting of dark-operative protochlorophyllide oxidoreductase, BchF, and BchC, with chlorin 3-hydroxyethyl chlorophyllide as a natural substrate identified for BchC. Modification of the isocyclic E ring, omission of the central magnesium ion, zinc as an alternative metal ion, and a non-reduced B ring system are tolerated by BchC. According to this broadened in vitro activity, the chlorin 3-hydroxyethyl chlorophyllide a is identified as a natural substrate of BchC in a reconstituted pathway consisting of dark-operative protochlorophyllide oxidoreductase, BchF, and BchC. The established reaction sequence allows for an additional new branching point for the synthesis of bacteriochlorophyll a |
-, 748192 |