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Results 1 - 3 of 3
EC Number Protein Variants Commentary Reference
Display the reaction diagram Show all sequences 4.2.1.165more bchF deletion mutant, mutant phenotype with altered pigment biosynthesis, detailed overview -, 748684
Display the reaction diagram Show all sequences 4.2.1.165more deletion of gene bchF in a BChl b-producing strain of Rhodobacter sphaeroides leads to the production of an analogue of bacteriochlorophyllide g, BChl gP, where P is phytyl, rather than the native BChl aP. In the bchF-deletion mutant, hydration of the C3-vinyl group in 3-vinyl bacteriochlorophyllide a is blocked, but this precursor can be esterified with phytol. Enzyme BchF deletion- in the DELTAbciA/bchXYZBv background results in formation of BChl g esterified with phytol (BChl gP) 747177
Display the reaction diagram Show all sequences 4.2.1.165more reconstitution of a bacteriochlorophyll formation pathway consisting of dark-operative protochlorophyllide oxidoreductase, BchF, and BchC, with chlorin 3-hydroxyethyl chlorophyllide as a natural substrate identified for BchC. Modification of the isocyclic E ring, omission of the central magnesium ion, zinc as an alternative metal ion, and a non-reduced B ring system are tolerated by BchC. According to this broadened in vitro activity, the chlorin 3-hydroxyethyl chlorophyllide a is identified as a natural substrate of BchC in a reconstituted pathway consisting of dark-operative protochlorophyllide oxidoreductase, BchF, and BchC. The established reaction sequence allows for an additional new branching point for the synthesis of bacteriochlorophyll a -, 748192
Results 1 - 3 of 3