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Results 1 - 9 of 9
EC Number Protein Variants Commentary Reference
Show all pathways known for 4.2.1.118Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.118E356A site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme 748670
Show all pathways known for 4.2.1.118Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.118H168A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme 748670
Show all pathways known for 4.2.1.118Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.118more gene asbF is unstable at 37°C, structure-based design to identify stabilizing mutations and creation a combinatorial library based upon predicted mutations at specific locations on the enzyme surface. A diversified asbF library (with about 2000 variants) is expressed in Escherichiaxa0coli harboring a GFP reporter system linked to the product of AsbF activity (3,4-dihydroxybenzoate, DHB) 746603
Show all pathways known for 4.2.1.118Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.118more quiC1 transposon quiC1 gene knockout in strain PAO1, recombinant plasmid-based expression of gene quiC1 from Pseudomonas putida QuiC1 in the Pseudomonas aeruginosa quiC1 knockout strain 748670
Show all pathways known for 4.2.1.118Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.118more the isolated N-terminal domain shows about 1/3 of the wild-type enzyme activity 748670
Show all pathways known for 4.2.1.118Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.118R207A site-directed mutagenesis, the mutant shows moderately reduced activity compared to the wild-type enzyme 748670
Show all pathways known for 4.2.1.118Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.118R210A site-directed mutagenesis, inactive mutant 748670
Show all pathways known for 4.2.1.118Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.118S206A site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme 748670
Show all pathways known for 4.2.1.118Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.118T61N/H135Y/H257P random mutagenesis, triple AsbF mutant Mut1, half-life of Mut1 at 37°C is over 10fold higher compared to wild-type AsbF. The second-order rate constants for both wild-type AsbF and Mut1 are approximately equal, thus demonstrating protein thermostability does not come at the expense of enzyme thermophilicity 746603
Results 1 - 9 of 9