EC Number |
Protein Variants |
Reference |
---|
4.2.1.118 | E356A |
site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme |
748670 |
4.2.1.118 | H168A |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme |
748670 |
4.2.1.118 | more |
gene asbF is unstable at 37°C, structure-based design to identify stabilizing mutations and creation a combinatorial library based upon predicted mutations at specific locations on the enzyme surface. A diversified asbF library (with about 2000 variants) is expressed in Escherichiaxa0coli harboring a GFP reporter system linked to the product of AsbF activity (3,4-dihydroxybenzoate, DHB) |
746603 |
4.2.1.118 | more |
quiC1 transposon quiC1 gene knockout in strain PAO1, recombinant plasmid-based expression of gene quiC1 from Pseudomonas putida QuiC1 in the Pseudomonas aeruginosa quiC1 knockout strain |
748670 |
4.2.1.118 | more |
the isolated N-terminal domain shows about 1/3 of the wild-type enzyme activity |
748670 |
4.2.1.118 | R207A |
site-directed mutagenesis, the mutant shows moderately reduced activity compared to the wild-type enzyme |
748670 |
4.2.1.118 | R210A |
site-directed mutagenesis, inactive mutant |
748670 |
4.2.1.118 | S206A |
site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme |
748670 |
4.2.1.118 | T61N/H135Y/H257P |
random mutagenesis, triple AsbF mutant Mut1, half-life of Mut1 at 37°C is over 10fold higher compared to wild-type AsbF. The second-order rate constants for both wild-type AsbF and Mut1 are approximately equal, thus demonstrating protein thermostability does not come at the expense of enzyme thermophilicity |
746603 |