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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.115C103M site-directed mutagenesis, the mutant is inactive, dimerization is prevented but the secondary structure is not significantly affected 692961
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.115C118M site-directed mutagenesis, not recombinantly expressable mutant 692961
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.115D126N site-directed mutagenesis, the mutant shows about 100fold lower activity with UDP-GlcNAc and with UDP-6-deoxy-6-fluoro-GlcNAc for HF elimination compared to the wild-type enzyme. Upon addition of UDP-4-keto-6-deoxy-GlcNAc to D126N the tightly bound NADPH is immediately oxidized 691202
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.115D149K/K150A site-directed mutagenesis, the mutant is inactive 692961
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.115D149K/K150D site-directed mutagenesis, the mutant is inactive 692961
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.115D70A site-directed mutagenesis, the mutant is inactive 692961
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.115D70N site-directed mutagenesis, not recombinantly expressable mutant 692961
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.115F78W site-directed mutagenesis of a cofactor binding residue, the mutant shows similar activity compared to the wild-type enzyme 729735
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.115G20A site-directed mutagenesis, the mutant is inactive 692961
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.115H86A site-directed mutagenesis, insoluble protein, the mutant shows reduced activity compared to the wild-type enzyme 692961
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