EC Number |
Protein Variants |
Reference |
---|
4.1.99.14 | C140A |
site-directed mutagenesis |
-, 747506 |
4.1.99.14 | C140A |
site-directed mutagenesis, mutant enzyme activity analysis using an SP dinucleoside and an SP-containing oligonucleotide |
-, 748852 |
4.1.99.14 | C140A |
site-directed mutagenesis, the mutant shows a similar protein and substrate binding structure compared to the wild-type enzyme, but 2.5fold reduced repair activity |
-, 728401 |
4.1.99.14 | C140A |
site-directed mutagenesis, the mutant still shows catalytic turnover, but reduced activity, due to a replacement of the thiyl radical by a thermodynamically comparable glycyl radical16 in the catalytic cycle |
727295 |
4.1.99.14 | C140A/S76C |
site-directed mutagenesis |
-, 747506 |
4.1.99.14 | C140A/S76C |
site-directed mutagenesis, contrary to the C140A mutant, the double mutant repaires the DNA lesion without generating DNA adducts. Furthermore, in the crystal structure, the mutant S76C is shown to be at 4.1 A from the methylene bridge of SP which is a shorter distance than the one reported for the wild-type enzyme |
-, 748852 |
4.1.99.14 | C140G |
site-directed mutagenesis, the mutant still shows catalytic turnover, but reduced activity, due to a replacement of the thiyl radical by a thermodynamically comparable glycyl radical16 in the catalytic cycle |
727295 |
4.1.99.14 | C140S |
site-directed mutagenesis, mutant enzyme activity analysis using an SP dinucleoside and an SP-containing oligonucleotide |
-, 748852 |
4.1.99.14 | C140S |
site-directed mutagenesis, the mutant shows a similar protein and substrate binding structure compared to the wild-type enzyme, but reduced repair activity |
-, 728401 |
4.1.99.14 | C141A |
site-directed mutagenesis, mutant enzyme activity analysis using an SP dinucleoside and an SP-containing oligonucleotide |
-, 748852 |