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EC Number
Protein Variants
Commentary
Reference
4.1.3.36
D163A
loss of the ligand binding ability
714260
4.1.3.36
D163E
14% 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity
714260
4.1.3.36
D163N
no 1,4-dihydroxy-2-naphthoyl-CoA synthetic activity
714260
4.1.3.36
D185E
reduced kcat
714273
4.1.3.36
D185G
no activity
714273
4.1.3.36
D185N
reduced kcat
714273
4.1.3.36
D192N
no activity
714273
4.1.3.36
F270A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
728604
4.1.3.36
G156D
no activity
714273
4.1.3.36
K89A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
728604
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