EC Number   |
Protein Variants |
Reference |
|---|
   4.1.1.82 | D258A |
9% of wild-type kcat for 3-phosphonopyruvate |
659267 |
| 4.1.1.82 | D260A |
0.1% of wild-type kcat for 3-phosphonopyruvate |
659267 |
| 4.1.1.82 | D297E |
catalytic turnover rate is 1.5fold less and Km is increased about 13fold compared to the wild type enzyme |
705200 |
| 4.1.1.82 | D297N |
catalytic turnover rate of D297N is 2fold lower and Km is increased 940fold compared to the wild type enzyme |
-, 705200 |
| 4.1.1.82 | E213A |
5% of wild-type kcat for 3-phosphonopyruvate |
659267 |
| 4.1.1.82 | G94A |
compared to wild type PPDC, Km is increased only 2fold, while kcat is 4fold lower |
-, 705200 |
| 4.1.1.82 | H110A |
no detectable activity |
-, 705200 |
| 4.1.1.82 | H111A |
the mutation has no significant influence on the steady state kinetic constants compared to the wild type enzyme |
-, 705200 |
| 4.1.1.82 | S25D |
catalytic turnover rate is 4fold less and Km is increased 460fold compared to the wild type enzyme |
-, 705200 |
| 4.1.1.82 | S25N |
catalytic turnover rate is 1.5fold less and Km is increased 80fold compared to the wild type enzyme |
705200 |
