EC Number   |
Protein Variants |
Reference |
|---|
   3.7.1.8 | C261A |
2fold decreased turnover rate, Cys-261 seems to be not involved in catalysis |
672081 |
| 3.7.1.8 | D237N |
reduced activity |
672080 |
| 3.7.1.8 | D247A |
mutant enzyme retains 29.93% enzyme activity |
755881 |
| 3.7.1.8 | F173D |
1.5fold increased Km value, 100fold decreased turnover rate |
672081 |
| 3.7.1.8 | F173G |
8fold increased Km value, 3.5fold decreased turnover rate |
672081 |
| 3.7.1.8 | H114A |
reduced activity, is able to accept the 6-phenyl-containing substrate, on a shorter time scale |
672080 |
| 3.7.1.8 | H263A |
overal structure similar, but asymmetry of the enzyme dimer more pronounced than for the native enzyme |
672081 |
| 3.7.1.8 | H265A |
reduced activity |
672080 |
| 3.7.1.8 | H265A |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme |
718941 |
| 3.7.1.8 | H265Q |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme and does not show formation of the carbanion catalytic intermediate in contrast to the wild-type enzyme |
719664 |
