EC Number   |
Protein Variants |
Reference |
|---|
   3.5.2.B2 | C111A |
(+)-gamma-lactamase activities is totally lost |
752562 |
| 3.5.2.B2 | C118A |
complete loss of activity |
735550 |
| 3.5.2.B2 | C145A |
mutant enzyme retains 51% of activities relative to the wild-type enzyme |
-, 752574 |
| 3.5.2.B2 | C145A |
mutation in catalytic triad, complete loss of activity |
-, 735762 |
| 3.5.2.B2 | C167A |
complete loss of gamma-lactamase activity |
-, 752580 |
| 3.5.2.B2 | D13A |
mutant enzyme retains 10% (+)-gamma-lactamase activity |
752562 |
| 3.5.2.B2 | D25A |
85% loss of gamma-lactamase activity |
-, 752580 |
| 3.5.2.B2 | D9A |
complete loss of activity |
735550 |
| 3.5.2.B2 | E95K/V54S |
optimal temperature is 10°C higher compared to wild-type enzyme. Mutant enzyme may be suitable for preparation of the optically pure intermediate (-)-Vince lactam. |
752551 |
| 3.5.2.B2 | F110A/V112G |
mutant enzyme shows no activity with (-)-Vince lactam |
752551 |
