EC Number   |
Protein Variants |
Reference |
|---|
    3.5.1.6 | A85E |
expression of the A85E plasmid results in severely reduced BUP-1 enzyme activity, with only 2.7% activity relative to the wild-type UPB1 plasmid |
689360 |
| 3.5.1.6 | C233A |
inactive mutant enzyme |
752722 |
| 3.5.1.6 | E137K |
inactive mutant enzyme |
752722 |
| 3.5.1.6 | E159A |
0.09% of the wild-type activity |
687655 |
| 3.5.1.6 | E159D |
0.09% of the wild-type activity |
687655 |
| 3.5.1.6 | G235R |
naturally occuring mutation and site-directed mutagenesis, inactive mutant. Mutation G235R introduces a large amino acid side chain for which there is no space available at this location. The larger structural rearrangements in the active site cavity required to prevent clashes with surrounding residues are expected to lead to enzyme inactivity and misfolding and defects in oligomerization, inability to obtain significant expression of soluble protein for this mutant |
733382 |
| 3.5.1.6 | G31G |
a naturally occuring synonymous mutation c.93C>T |
734810 |
| 3.5.1.6 | H226E |
no activity |
687655 |
| 3.5.1.6 | H262A |
8.9% of the wild-type activity |
687655 |
| 3.5.1.6 | H397N |
7.2% of the wild-type activity |
687655 |
