EC Number   |
Protein Variants |
Reference |
|---|
    3.5.1.4 | C118A |
complete loss of activity |
735550 |
| 3.5.1.4 | C141A |
site-directed mutagenesis, inactive mutant |
733190 |
| 3.5.1.4 | C145A |
mutant enzyme shows 9.7% of wild-type eactivity |
686659 |
| 3.5.1.4 | C145S |
mutant enzyme shows 6.2% of wild-type eactivity |
686659 |
| 3.5.1.4 | C166A |
mutant catalytically inactive |
209131 |
| 3.5.1.4 | C166S |
mutant catalytically inactive |
209131 |
| 3.5.1.4 | C249A |
inhibition of the mutant by ibuprofen is similar than that of wild type |
655133 |
| 3.5.1.4 | D133A |
inactive mutant enzyme |
686659 |
| 3.5.1.4 | D133E |
mutant enzyme shows 0.16% of wild-type eactivity |
686659 |
| 3.5.1.4 | D487N |
site-specific mutagenesis, the D487N mutation is located in the center of the protein, far distant from the 33-48 segment. The mutant amidase, like the wild-type enzyme, responds to changes in pH and temperature with a conformational change affecting the dimer-octamer equilibrium |
711039 |
