EC Number   |
Protein Variants |
Reference |
|---|
    3.5.1.19 | C133A |
site-directed mutagenesis, the mutation affectes the active site cavity, and enhances the binding of pyrazinamide compared to the wild-type enzyme |
-, 735084 |
| 3.5.1.19 | C136A |
the mutant is unable to catalyze nicotinamide hydrolysis |
718859 |
| 3.5.1.19 | C136S |
inactive |
718883 |
| 3.5.1.19 | C136S |
the mutant is unable to catalyze nicotinamide hydrolysis |
718859 |
| 3.5.1.19 | C138A |
significant decrease in enzyme activity |
679806 |
| 3.5.1.19 | C161A |
inactive |
-, 719619, 720499 |
| 3.5.1.19 | C167A |
site-directed mutagenesis, inactive mutant, no rescue the inhibition of NAD+-dependent histone deacetylase, i.e. HDAC, activity of Sir2 by nicotinamide |
670237 |
| 3.5.1.19 | C167A |
the mutant shows most strongly decreased kcat value compared to the wild type enzyme (below the assay detection limit) |
718924 |
| 3.5.1.19 | D49A |
significant decrease in enzyme activity |
679806 |
| 3.5.1.19 | D51A |
the zinc-binding mutant shows decreased kcat value compared to the wild type enzyme |
718924 |
