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EC Number	Protein Variants	Commentary	Reference
3.4.24.25	more	isolation of a vibriolysin mutant by in vivo random mutagenensis. contrary to wild-type, the mutant is expressed as active mature vibriolysin in Escherichia coli. The N-terminal propeptide of the engineered enzyme is processed and degraded, confirming that the propeptide inhibits the mature enzyme. Two mutations result in the substitution of stop codon for Trp at position 11 in the signal peptide and of Val for Ala at position 183 in the N-terminal propeptide	701058

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Release 2023.1 (January 2023)