EC Number   |
Protein Variants |
Reference |
|---|
   3.4.22.64 | C254A |
enzymatically inactive |
754299 |
| 3.4.22.64 | D285A |
mutation disrupts the processing site in the linker region between the large and small subunits of caspase-11 |
754299 |
| 3.4.22.64 | more |
all Casp1-/- mice also lack caspase-11, due to the generation of the Casp1-/- line in the 129 mouse strain background, which express a mis-spliced and truncated version of the Casp11 messenger RNA |
731611 |
| 3.4.22.64 | more |
all Casp1-/- mouse strains generated from 129 embryonic stem cells also lack caspase-11 due to a 5-bp deletion in the caspase-11 locus that causes loss of the catalytic domain. As the caspase-11 and caspase-1 loci are located physically close together, the mutations do not segregate during backcrossing, leading to double mutant mice |
731744 |
| 3.4.22.64 | more |
enzyme downregulation by siRNA |
732944 |
| 3.4.22.64 | more |
enzyme downregulation by siRNA and shRNA sequence targeting caspase-11 |
-, 732944 |
| 3.4.22.64 | more |
generation of Casp11-/- knockout mice, overview. The common mouse knockout strategy is to inject genetically targeted 129-derived ES cells into host C57BL/6 blastocysts and then backcross the chimera with other strains, commonly C57BL/6, for over 10 generations |
731905 |
| 3.4.22.64 | more |
generation of Casp11-/- mice, all existing caspase-1 deficient mice also lack Caspase-11 due to the backcrossing of a mutant Casp11 allele from 129 into C57BL/6 mice |
-, 732915 |
| 3.4.22.64 | more |
recombination and segregation of the nonfunctional Casp11 gene away from the Casp1-/- locus is nearly impossible because Casp1 and Casp11 occur only about 1500 bp apart on chromosome 9 in mice |
731613 |
| 3.4.22.64 | more |
targeting Casp11 exon 5 for deletion in C57BL/6 embryonic stem cells. Strain 129 mice, like Casp11-/- mutant mice, exhibits defects in IL-1beta production and harbours a mutation in the Casp11 locus that attenuates caspase-11 expression |
-, 718162 |
