EC Number   |
Protein Variants |
Reference |
|---|
   3.4.22.63 | C401G |
active site, essential for CASP10-induced cell death |
696452 |
| 3.4.22.63 | D219A |
involved in proteolytic activation, essential for CASP10-induced cell death |
696452 |
| 3.4.22.63 | D297A |
site-directed mutagenesis, the single mutation D297A completely abrogates autocleavage between the subunits, the purified mutant is a single chain of 35 kDa. The cleavage site mutant has restricted specificity and highly reduced activity on protein substrates, overview |
717252 |
| 3.4.22.63 | D416A |
involved in proteolytic activation, essential for CASP10-induced cell death |
696452 |
| 3.4.22.63 | I406L |
impairs apoptosis when transfected alone and dominantly inhibits apoptosis mediated by wild-type caspase-10 in a co-transfection assay |
665128 |
| 3.4.22.63 | L285F |
impairs apoptosis when transfected alone and dominantly inhibits apoptosis mediated by wild-type caspase-10 in a co-transfection assay |
665128 |
| 3.4.22.63 | L285P |
the mutation may contribute to the pathogenesis of factions of T-acute lymphoblastic leukemia and multiple myeloma |
710208 |
| 3.4.22.63 | more |
caspase-10 isoforms are transiently downregulated using siRNAs targeting all caspase-10 isoforms, or siRNAs specific for individual caspase-10 isoforms. Downregulation of individual or all caspase-10 isoforms in SH-EP cells does not affect TRAIL sensitivity |
717459 |
| 3.4.22.63 | more |
construction of a truncated mutant lacking the first 202 residues, and of the truncation mutant with a substitution D297A in the catalytic site, cloning into a vector with chimeric protein with an N-terminal His-tag followed by the Fv-domain. Chemically inducible dimerization fusions activate the wild-type but not the cleavage site mutant caspase-10 |
717252 |
| 3.4.22.63 | more |
somatic mutation of caspase-10 is rare in colon, breast, lung, and hepatocellular carcinomas, caspase-10 mutation may contribute to the pathogenesis of some colon carcinomas |
710209 |
