EC Number   |
Protein Variants |
Reference |
|---|
   3.4.22.48 | C21A |
purification of native staphopain B unsuccessful since strain SW035 produces only minimally detectable protease activity, active-site mutation generated by site-directed mutagenesis, catalytically inactive mutant StpBwar C21A used for interaction studies |
683202 |
| 3.4.22.48 | C237A |
inactive ScpA mutant |
-, 731911 |
| 3.4.22.48 | C243A |
inactive SspB mutant |
-, 731911 |
| 3.4.22.48 | more |
construction of an inactive mutant, active site mutation of staphopain A results in abrogation of production of both co-expressed proteins |
667487 |
| 3.4.22.48 | more |
exchange of active site cysteine residue for an alanine, inactive mutant, fusion to N-terminal His-tag |
643962 |
| 3.4.22.48 | more |
inactivation of gene sspA by transposon insertion exertes a polar effect on genes sspB and sspC expression, the inactivation of all three genes results in attenuation of virulence |
667029 |
