EC Number   |
Protein Variants |
Reference |
|---|
   3.4.21.9 | C112S |
site-directed mutagenesis, replacement of the free cysteine residue with serine improves the refolding yield of the recombinant enzyme by 50%. The heat stability of this C112S variant was also significantly improved by supercharging |
718352 |
| 3.4.21.9 | E136Y |
the mutant shows clearly reduced activity compared to the wild type enzyme |
732855 |
| 3.4.21.9 | E136Y/R213L |
the mutant shows about wild type activity |
732855 |
| 3.4.21.9 | E173A |
site directed mutagenesis |
716743 |
| 3.4.21.9 | E173K |
site directed mutagenesis |
716743 |
| 3.4.21.9 | F144A |
site directed mutagenesis |
716743 |
| 3.4.21.9 | F144S |
site directed mutagenesis |
716743 |
| 3.4.21.9 | H24Q |
the mutant shows slightly reduced activity compared to the wild type enzyme |
732855 |
| 3.4.21.9 | K63A |
site directed mutagenesis |
716743 |
| 3.4.21.9 | K63E |
site directed mutagenesis |
716743 |
