EC Number   |
Protein Variants |
Reference |
|---|
   3.4.21.88 | G126D |
site-directed mutagenesis, cleavage site mutant |
-, 752390 |
| 3.4.21.88 | G85D |
change in cleavage site, that blocks autocleavage but has normal active site, crystallization data |
664539 |
| 3.4.21.88 | G85D |
LexA mutant |
652581 |
| 3.4.21.88 | G94E |
prevents cleavage of the LexA |
683903 |
| 3.4.21.88 | K156A |
site directed mutagenesis |
683784 |
| 3.4.21.88 | K197A |
site-directed mutagenesis, active site mutant |
-, 752390 |
| 3.4.21.88 | L89P/Q92W/E152A/K156A |
designed to trap protein in conformation required for cleavage. Crystallization data of tryptic fragment containing amino acids 68202 |
664539 |
| 3.4.21.88 | more |
construction of a lexA deletion strain WP3DELTAlexA, no growth defect is observed. 481 and 108 genes are differentially expressed at 20°C and 4°C, respectively, as demonstrated by comparative whole genome microarray analysis |
-, 753674 |
| 3.4.21.88 | more |
construction of knockout mutant DELTAlexA mutant, the decrease in gene transfer frequency from the DELTAlexA mutant is associated with a decrease in transcription of the RcGTA structural gene cluster, the -galactosidase activities of WTand DELTAlexA mutant cells containing a plasmid with the RcGTA promoter fused to lacZ are compared. RcGTA recipient capability is decreased in the DELTAlexA mutant, phenotype, and morphology of DELTAlexA mutant cells, overview |
-, 754015 |
| 3.4.21.88 | more |
construction of strain BW27784 sulA6209 lexA71, that lacks LexA activity |
708930 |
