EC Number   |
Protein Variants |
Reference |
|---|
   3.4.21.81 | more |
introduction of site-specific disulfide bonds into the flexible N-terminal loop of natural Kazal-type inhibitors to characterize the thermodynamics of the reactive site peptide bond hydrolysis. Mutant P14C/N39C is a disulfide variant of the ovomucoid third domain from silver pheasant carrying an engineered Cys14Cys39 bond near the reactive site. The conversion rate of P14C/N39C by streptogrisin B is much faster than that for wild type under any pH condition |
718026 |
| 3.4.21.81 | S195A |
active-site variant of protease B with peptide-ligation activity |
650820 |
| 3.4.21.81 | S195A/T213L/F228H |
i.e. streptoligase, catalyzes peptide ligation efficiently. Ligation proceeds via an acyl-enzyme intermediate involving H57. Mutant exhibits half-life for unfolding of 16.3 min at 55°C in the absence of stabilizing substrates |
664042 |
| 3.4.21.81 | S195C |
active-site variant of protease B with peptide-ligation activity |
650820 |
| 3.4.21.81 | S195G |
active-site variant of protease B with peptide-ligation activity |
650820 |
| 3.4.21.81 | S195G/H57N |
active-site variant of protease B with peptide-ligation activity |
650820 |
