EC Number   |
Protein Variants |
Reference |
|---|
   3.4.21.117 | analysis |
ELISA-type immunoassay for human kallikrein quantification |
664621 |
| 3.4.21.117 | H41F |
mutant is inhibited by Zn2+ as the wild type enzyme |
682553 |
| 3.4.21.117 | H41F |
site-directed mutagenesis, the mutant is inhibited by Zn2+ like the wild-type enzyme |
682553 |
| 3.4.21.117 | H99A |
mutant exhibits virtually no Zn2+ inhibition |
682553 |
| 3.4.21.117 | H99A |
site-directed mutagenesis, the mutant shows resistance against inhibition by Zn2+ in contrast to the wild-type enzyme |
682553 |
| 3.4.21.117 | more |
KLK7 is overexpressed in the pancreatic cancer cell line BxPC-3, and in vitro cell adhesion assays are performed to determine alterations in cell adhesion to collagen I, fibronectin, and vitronectin compared with vector-transfected cells. Changes in cell surface expression of alphavbeta3 integrin are examined by flow cytometry, and the release of soluble urokinase-type plasminogen activator receptor (uPAR) is monitored by Western blot analysis. The expression of KLK7 in BxPC-3 cells leads to reduced adhesion to vitronectin but not collagen I or fibronectin. Loss of cell adhesion to vitronectin is not caused by changes in the level of alphavbeta3 integrin cell surface expression. A significant increase in the amount of uPAR shed is observed in KLK7-expressing cells compared with vector-transfected control cells |
700554 |
| 3.4.21.117 | more |
the individual risk of eczema conferred by the SPINK5 polymorphism rs2303067 (Glu420Lys) and a previously described insertion in the 3'-untranslated region of KLK7 and potential epistatic effects between these variants and filaggrin mutations are analysed in patients with eczema and healthy counterparts. No association is seen with the SPINK5 or KLK7 variants. A weaker effect is observed for the SPINK5 variant with maternal transmission in the family-based study |
698443 |
| 3.4.21.117 | S195A |
mutation in catalytic triad, shows residual catalytic activity |
755479 |
| 3.4.21.117 | S195A/D102N |
inactive |
755479 |
