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Results 1 - 10 of 14 > >>
EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.15.6more coexpression of cyanophycin and CGPase in the same plant without substrate degradation in planta by transient expression of CGPase CphB either in the plastid or cytosol, or the Pseudomonas alcaligenes CGPase CphE in the cytosol of CGP-producing Nicotiana tabacum plants. No cyanophycin degradation appears prior to cell homogenization independent of the CGPase produced. In crude plant extracts, only cytosolic CphE leads to a fast degradation of cyanophycin. CphE also shows higher stability and in vitro activity compared to both CphB variants 755615
Display the word mapDisplay the reaction diagram Show all sequences 3.4.15.6more Gly101, Asp172, Gly173, Arg178, Arg180 and Arg183 form a conserved pocket adjacent to the catalytic Ser132, these are functionally critical residues. The cyanophycinase active site contains three absolutely conserved Arg residues (Arg178, Arg180 and Arg183), which line up along one side of the pocket 709525
Display the word mapDisplay the reaction diagram Show all sequences 3.4.15.6more optimized transient expression in Nicotiana benthamiana plants to produce high amounts of active CGPase. Protein stability is increased by the translational fusion of CGPase to the green fluorescent protein or to the transit peptide of the small subunit of RuBisCO for peptide production in the chloroplasts 755028
Display the word mapDisplay the reaction diagram Show all sequences 3.4.15.6N100A site-directed mutagenesis, 40% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C 709525
Display the word mapDisplay the reaction diagram Show all sequences 3.4.15.6N158A site-directed mutagenesis, 41% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C 709525
Display the word mapDisplay the reaction diagram Show all sequences 3.4.15.6N172A site-directed mutagenesis, 0.02% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C 709525
Display the word mapDisplay the reaction diagram Show all sequences 3.4.15.6N17A site-directed mutagenesis, 60% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C 709525
Display the word mapDisplay the reaction diagram Show all sequences 3.4.15.6N202A site-directed mutagenesis, 8% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C 709525
Display the word mapDisplay the reaction diagram Show all sequences 3.4.15.6Q101A site-directed mutagenesis, 0.02% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C 709525
Display the word mapDisplay the reaction diagram Show all sequences 3.4.15.6Q173A site-directed mutagenesis, 0.5% activity of the wild-type, measured with 75 microg/ml of [L-Asp(4-Arg)]n in phosphate buffer at pH 8.3 and 30°C 709525
Results 1 - 10 of 14 > >>