EC Number   |
Protein Variants |
Reference |
|---|
   3.4.13.20 | C102S |
efficiency for carnosine degradation is not influenced by the addition of cysteine |
754284 |
| 3.4.13.20 | C229S |
addition of cysteine to C229S significantly reduces CN1 catalytic efficiency |
754284 |
| 3.4.13.20 | D166A |
mutation in putative metal binding site, complete loss of activity |
683621 |
| 3.4.13.20 | E201A |
mutation in putative metal binding site, complete loss of activity |
683621 |
| 3.4.13.20 | E201A/D229A |
mutation in putative metal binding site, complete loss of activity |
683621 |
| 3.4.13.20 | G249A |
the precursor is not processeed at the Gly249-Ser250 peptide bond. Production of an uncleaved and inactive protein |
-, 707418 |
| 3.4.13.20 | G249D |
the precursor is not processeed at the Gly249-Ser250 peptide bond. Production of an uncleaved and inactive protein |
-, 707418 |
| 3.4.13.20 | H133A |
mutation in putative metal binding site, complete loss of activity |
683621 |
| 3.4.13.20 | H228A |
inactive |
698808 |
| 3.4.13.20 | more |
expression of constructs varying in CTG repeats coding for 4,5,6,7,or 8 leucines in the signal peptide. Enzyme secretion is significantly higher in cells expressing variants with more than five leucins in the signal peptide |
683393 |
