EC Number |
Protein Variants |
Reference |
---|
3.4.13.19 | D22H |
mutant with no residual enzyme acitvity |
707533 |
3.4.13.19 | D320A |
mutant with no residual enzyme acitvity |
707533 |
3.4.13.19 | D320N |
mutant with no residual enzyme acitvity |
707533 |
3.4.13.19 | H128L |
mutant enzyme exhibits a specific activity and Km for Gly-D-Phe comparable with that of the wild-type enzyme |
647114 |
3.4.13.19 | H150A |
mutant with more than doubled Km-values |
707533 |
3.4.13.19 | H150N |
mutant with more than doubled Km-values |
707533 |
3.4.13.19 | H152L |
inactive mutant enzyme is expressed at the cell surface at equivalent levels to the wild-type, the mutant enzyme fails to bind to cilastatin-Sepharose in contrast to the wild-type enzyme |
647114 |
3.4.13.19 | H198K |
inactive mutant enzyme is expressed at the cell surface at equivalent levels to the wild-type |
647114 |
3.4.13.19 | H20L |
inactive mutant enzyme is expressed at the cell surface at equivalent levels to the wild-type |
647114 |
3.4.13.19 | H413K/H417K |
catalytic activity reduced to background levels. Animals carrying the mutant enzyme generate 1 embryo from 22 plugged females compared to 153 embryos from 19 females in wild-type. There is no difference in sperm count, morphology or motility after capacitation in mutant animals. While sperm from wild-type males readily binds to the zona pellucida of unfertilized eggs, sperm from homozygous mutant males shows background levels of binding |
670357 |