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Results 1 - 10 of 10
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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.11.21H170F inactive mutant enzyme 649470
Display the word mapDisplay the reaction diagram Show all sequences 3.4.11.21H33F mutant enzyme with decreased turnover number 649470
Display the word mapDisplay the reaction diagram Show all sequences 3.4.11.21H349F mutant enzyme with decreased turnover number 649470
Display the word mapDisplay the reaction diagram Show all sequences 3.4.11.21H352F dramatically reduced activity, destabilization of quarternary structure and dissociation of the native 440000 Da enzyme 649470
Display the word mapDisplay the reaction diagram Show all sequences 3.4.11.21H359F mutant enzyme with decreased turnover number 649470
Display the word mapDisplay the reaction diagram Show all sequences 3.4.11.21H363F mutant enzyme with decreased turnover number 649470
Display the word mapDisplay the reaction diagram Show all sequences 3.4.11.21H440F inactive mutant enzyme 649470
Display the word mapDisplay the reaction diagram Show all sequences 3.4.11.21H94F inactive mutant enzyme 649470
Display the word mapDisplay the reaction diagram Show all sequences 3.4.11.21more knockout of enzyme TgAAP using the CRISPR/Cas9 knockout system, knockout plasmid pCD-TgAAP is constructed by inserting DHFR coding sequence and TgAAP-specific single guide RNA (gRNA) into plasmid pSAG1::CAS9-U6::sgUPRT, which contains Cas9, nuclear localization signal (NLS), and expresses GFP fusion element. The substrate activity is almost completely lost in the DELTATgAAP strain, thus, no other enzyme complements the TgAAP activity -, 755445
Display the word mapDisplay the reaction diagram Show all sequences 3.4.11.21more random insertional mutagenesis, the ape4 mutants are sensitive to high temperature growth. ape4 Mutation affects multiple virulence factors in Cryptococcus neoformans -, 755145
Results 1 - 10 of 10
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